Dental and periodontal phenotype in sclerostin knockout mice.
Dental and periodontal phenotype in sclerostin knockout mice.
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DOI：
10.1038/ijos.2014.12
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发表时间：
2014-06
影响因子：
14.9
通讯作者：
Gruber, Reinhard
中科院分区：
文献类型：
--
作者：
Kucher, Ulrike;Schwarze, Uwe Y.;Dobsak, Toni;Heimel, Patrick;Bosshardt, Dieter D.;Kneissel, Michaela;Gruber, Reinhard
硬化蛋白是一种控制骨代谢的Wnt信号传导拮抗剂。硬化蛋白由骨细胞和牙骨质细胞表达，然而，其在牙齿结构形成中的作用仍不清楚。在这里，我们分析了sclerostin基因敲除小鼠的下颌骨，以确定sclerostin对牙齿结构和尺寸的影响，使用组织形态计量学和微计算机断层扫描（μCT）成像。对缺乏功能性硬化素基因的小鼠和野生型对照小鼠的第一下磨牙及其周围结构进行μCT和组织形态计量学分析。每组6只动物的μCT显示，在sclerostin敲除小鼠中，基底骨以及牙槽部分的冠状部和顶端部的尺寸增加。牙齿和髓室体积无显著差异。对四只野生型和三只sclerostin基因敲除小鼠的描述性组织形态计量学分析表明，牙骨质宽度增加，牙周间隙宽度随之适度减少。总之，这些结果表明，sclerostin的缺乏主要改变了骨和牙骨质的表型，而不是在牙齿结构如牙本质中产生异常。
Sclerostin is a Wnt signalling antagonist that controls bone metabolism. Sclerostin is expressed by osteocytes and cementocytes; however, its role in the formation of dental structures remains unclear. Here, we analysed the mandibles of sclerostin knockout mice to determine the influence of sclerostin on dental structures and dimensions using histomorphometry and micro-computed tomography (μCT) imaging. μCT and histomorphometric analyses were performed on the first lower molar and its surrounding structures in mice lacking a functional sclerostin gene and in wild-type controls. μCT on six animals in each group revealed that the dimension of the basal bone as well as the coronal and apical part of alveolar part increased in the sclerostin knockout mice. No significant differences were observed for the tooth and pulp chamber volume. Descriptive histomorphometric analyses of four wild-type and three sclerostin knockout mice demonstrated an increased width of the cementum and a concomitant moderate decrease in the periodontal space width. Taken together, these results suggest that the lack of sclerostin mainly alters the bone and cementum phenotypes rather than producing abnormalities in tooth structures such as dentin.
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影响因子：
4.1
作者：
Gluhak-Heinrich, J.;Guo, D.;Yang, W.;Harris, M. A.;Lichtler, A.;Kream, B.;Zhang, J.;Feng, J. Q.;Smith, L. C.;Dechow, P.;Harris, S. E.
通讯作者：
Harris, S. E.
影响因子：
5.8
作者：
Gardner, JC;van Bezooijen, RL;Papapoulos, SE
通讯作者：
Papapoulos, SE
影响因子：
6.2
作者：
Li, Xiaodong;Ominsky, Michael S.;Paszty, Chris
通讯作者：
Paszty, Chris
影响因子：
4.1
作者：
Niziolek, Paul J.;Farmer, Takeisha L.;Robling, Alexander G.
通讯作者：
Robling, Alexander G.
DOI：
10.1016/j.bbrc.2011.07.116
发表时间：
2011-09-09
影响因子：
3.1
作者：
Kim, Tak-Heun;Lee, Ju-Yeon;Cho, Eui-Sic
通讯作者：
Cho, Eui-Sic