High-Throughput Signal-On Photoelectrochemical Immunoassay of Lysozyme Based on Hole-Trapping Triggered by Disintegrating Bioconjugates of Dopamine-Grafted Silica Nanospheres
High-Throughput Signal-On Photoelectrochemical Immunoassay of Lysozyme Based on Hole-Trapping Triggered by Disintegrating Bioconjugates of Dopamine-Grafted Silica Nanospheres
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基于多巴胺接枝二氧化硅纳米球崩解生物共轭物引发的空穴捕获的溶菌酶高通量信号光电化学免疫分析
DOI：
10.1021/acssensors.8b00253
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发表时间：
2018-08-01
期刊：
影响因子：
8.9
通讯作者：
Gong, Jingming
中科院分区：
文献类型：
--
作者：
Li, Xin;Wang, Xinlei;Gong, Jingming
一个独特的分裂型光电化学（PEC）免疫分析已被构建用于检测低丰度的生物化合物（溶菌酶，Lyz，在这种情况下使用）通过一种新的触发策略，通过分解生物共轭多巴胺接枝的二氧化硅纳米球（DA@SiO（2）NS）的信号放大。DA@SiO（2）NS的优选电子供体组装体首先用作分子印刷板，用于通过酰胺反应定位抗Lyz二抗（Ab（2））。通过高结合微量板中的特异性免疫反应（夹心免疫测定），获得了基于DA@SiO（2）NS的生物缀合物。通过酸蚀刻引发生物缀合物的分解，释放出大量DA的电子供体，从而有效地触发空穴捕获，获得放大的信号。基于ZnIn 2S 4的异质结作为光活性材料的智能集成、分裂型检测模式以及通过分解基于DA@SiO（2）NS的生物缀合物的新触发策略提供了用于检测Lyz的有吸引力的高通量信号开启PEC免疫分析。这种不寻常的PEC传感器表现出突出的线性响应的浓度范围内0.002和500 ng mL（-1），和检测限低至0.6 ppt（S/N = 3）。所制造的测定具有成本效益和灵敏度。它已成功地用于测量Lyz在真实的样品，这表明了巨大的实际应用前景。
A unique split-type photoelectrochemical (PEC) immunoassay has been constructed for detection of low-abundance biocompounds (lysozyme, Lyz, used in this case) via a new trigger strategy by disintegrating bioconjugates of dopamine-grafted silica nanospheres (DA@SiO(2)NSs) for signal amplification. The preferred electron donor assembly of DA@SiO(2)NSs is first used as a molecular printboard for positioning anti-Lyz secondary antibody (Ab(2)) through an amide reaction. With specific immunoreactions in a high-binding microplate, a sandwich immunoassay, the DA@SiO(2)NSs-based bioconjugate is achieved. By initiating the disintegration of the bioconjugates via acid etching, numerous electron donors of DA are released, thus efficiently triggering hole-trapping with amplified signals obtained. The smart integration of ZnIn2S4-based heterojunctions as photoactive material, a split-type detection mode, and a new trigger strategy by disintegrating the DA@SiO(2)NSs-based bioconjugate offer an attractive high-throughput signal-on PEC immunoassay for detection of Lyz. Such an unusual PEC sensor exhibits an outstanding linear response to the concentration in the range between 0.002 and 500 ng mL(-1), and the detection limit is as low as 0.6 ppt (S/N = 3). The as fabricated assay is cost-effective and sensitive. It has been successfully used for measuring Lyz in real samples, which demonstrates great promise for practical applications.