Bacterial maximum non-inhibitory and minimum inhibitory concentrations of different water activity depressing solutes
Bacterial maximum non-inhibitory and minimum inhibitory concentrations of different water activity depressing solutes
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DOI：
10.1016/j.ijfoodmicro.2014.07.011
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发表时间：
2014-10-01
影响因子：
5.4
通讯作者：
Condon, S.
中科院分区：
文献类型：
--
作者：
Cebrian, G.;Arroyo, C.;Condon, S.
NaCl MNIC（最大非抑制浓度）和MIC（最小抑制浓度）的生长的各种菌株的6个细菌物种进行了测定，然后与其他7种溶质获得的比较。还评价了先前生长条件对MNIC和MIC的影响。在每个种属内研究的菌株之间未发现MNIC和MIC的显著变化。在所有调查的因素中，只有生长阶段-革兰氏阴性菌-和高NaCl浓度下的生长导致NaCl MNICs.Species的变化，可根据其NaCl MNICs和MIC（按降序排列）分类如下：金黄色葡萄球菌，单核细胞增生李斯特菌，坂崎克罗伊氏菌，屎肠球菌，大肠杆菌和鼠伤寒沙门氏菌。对于KCl、LiCl和乙酸钠，获得了类似的结果，但对于研究的其余溶质（蔗糖、甘油、MgCl 2和CaCl 2），没有获得类似的结果。所得结果表明，一般而言，革兰氏阴性菌对所有溶质的MNIC和MIC均低于革兰氏阳性菌。金黄色葡萄球菌是最耐受溶质的微生物。当在摩尔基础上进行比较时，甘油对所有微生物显示出最高的MNIC和MIC-除了S。aureus-和LiCl最低。NaCl MNIC和MIC与KCl相比，在摩尔基础上无显著差异。因此，NaCl的抑制作用与Na+的特异性作用无关。结果还表明，某些物种的Na+耐受性是Cl-依赖性的，而另一些则不是，LiCl、CaCl_2和MgCl_2的抑制作用与a（w）-减少以外的因素有关。(C)2014爱思唯尔有限公司版权所有。
The NaCl MNICs (maximum non-inhibitory concentrations) and MICs (minimum inhibitory concentrations) for growth of various strains of six bacterial species were determined and then compared with those obtained for seven other solutes. The influence of prior growth conditions on the MNICs and MICs was also evaluated. No significant changes on the MNICs and MICs were found among the strains studied within each species. Among all factors investigated, only growth phase -for Gram-negatives- and growth at high NaCl concentrations led to a change in the NaCl MNICs.Species could be classified depending on its NaCl MNICs and MICs (in decreasing order) as follows: Staphylococcus aureus, Listeria monocytogenes, Cronobacter sakazakii, Enterococcus faecium, Escherichia coli and Salmonella Typhimurium. Similar results were obtained for KCl, LiCl, and sodium acetate, but not for the remaining solutes investigated (sucrose, glycerol, MgCl2 and CaCl2). Results obtained indicate that, in general, Gram-negatives showed lower MNICs and MICs than Gram-positives for all the solutes, S. aureus being the most solute tolerant microorganism. When compared on a molar basis, glycerol showed the highest MNICs and MICs for all the microorganisms -except for S. aureus- and LiCl the lowest ones. NaCl MNICs and MICs were not significantly different from those of KCl when compared on a molar basis. Therefore, the inhibitory action of NaCl could not be linked to the specific action of Na+ Results also showed that the Na+ tolerance of some species was Cl- dependent whereas for others it was not, and that factors others than a(w)-decrease contribute to the inhibitory action of LiCl, CaCl2 and MgCl2. (C) 2014 Elsevier B.V. All rights reserved.