Evaluation of a flushing solution designed to protect kidneys from in situ ischemia.
Evaluation of a flushing solution designed to protect kidneys from in situ ischemia.
复制标题
评估旨在保护肾脏免受原位缺血的冲洗溶液。
DOI：
10.1016/s0272-6386(85)80038-x
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发表时间：
1985
期刊：
影响因子：
--
通讯作者：
Bates,SB
中科院分区：
文献类型：
--
作者：
Andrews,PM;Bates,SB
就以下方面评价原位冲洗溶液：（1）其在60、90和120分钟常温缺血期间保护肾脏的能力;（2）在冲洗溶液中使用细胞内与细胞外电解质组合物的效果;和（3）冲洗溶液补充原位低温作为长期缺血期间的保护措施的能力。用含有50毫渗摩尔蔗糖的等渗磷酸盐缓冲溶液（pH 7.2）原位短暂冲洗大鼠肾脏。然后立即夹住左肾蒂，使肾脏缺血，并将冲洗液保持在肾脏中。移除血管蒂钳后，进行右肾对侧肾切除术，并测定每日血清肌酐水平以评价缺血后肾功能。结果表明：（1）在常温缺血60分钟后，冲洗程序在预防缺血后急性肾衰竭方面非常有效，但对于90分钟或更长时间的缺血时间，其有效性相当低;（2）冲洗溶液中的细胞内电解质组合物不能改善该溶液的保护作用;以及（3）冲洗程序可以显著地改善由原位低温提供的保护。
An in situ flushing solution was evaluated with regard to the following: (1) its ability to protect the kidney during 60, 90, and 120 minutes of normothermic ischemia; (2) the effects of using an intracellular versus extracellular electrolyte composition in the flushing solution; and (3) the ability of the flushing solution to complement in situ hypothermia as a protective measure during long-term ischemia. Rat kidneys were briefly flushed in situ with an isotonic phosphate buffered solution (pH 7.2) containing 50 milliosmole of sucrose. The left renal pedicle was then immediately clamped to render the kidney ischemic and to hold the flushing solution in the kidney. Following removal of the pedicle clamp, a contralateral nephrectomy of the right kidney was performed and daily serum creatinine levels determined to evaluate postischemic renal function. The results indicate the following: (1) the flushing procedure is very effective in preventing postischemic acute renal failure following 60 minutes of normothermic ischemia, but is considerably less effective for ischemic times of 90 minutes or more; (2) an intracellular electrolyte composition in the flushing solution does not improve the protective effects of this solution; and (3) the flushing procedure can significantly improve on the protection otherwise provided by in situ hypothermia.