Localization of nucleophosmin in nuclear matrix and changes in its expression during the differentiation of human neuroblastoma induced by retinoic acid
Localization of nucleophosmin in nuclear matrix and changes in its expression during the differentiation of human neuroblastoma induced by retinoic acid
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DOI：
10.1002/jcb.22663
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发表时间：
2010-09
影响因子：
4
通讯作者：
Song-Lin Shi;Ying Liang;Qi-Fu Li;Qing-Rong Liu;Guang-Jun Jing;San-ying Wang;Xiuping Zhang;Fu-Yun Wu
中科院分区：
文献类型：
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作者：
Song-Lin Shi;Ying Liang;Qi-Fu Li;Qing-Rong Liu;Guang-Jun Jing;San-ying Wang;Xiuping Zhang;Fu-Yun Wu
在本文中，我们选择性地提取了经视黄酸（RA）处理前后的人神经母细胞瘤SK‐N‐SH细胞的核基质和中间丝系统。研究了核磷蛋白（nuclear ophosmin， NPM）在核基质中的分布及其与相关基因产物的共定位。二维凝胶电泳和MALDI - TOF结果显示，NPM是核基质的一个组成部分，其在RA处理后的SK - N - SH细胞中的表达下调。免疫荧光显微镜观察进一步表明，NPM定位于SK‐N‐SH细胞的核基质中，RA治疗后NPM的表达水平和分布发生改变。在激光共聚焦显微镜下观察到NPM与c - myc、c - fos、p53和Rb在SK - N - SH细胞中的共定位，但RA改变了共定位区域。我们的结果证明NPM是一种核基质蛋白，定位于核基质纤维中。NPM及其相关基因和癌基因的共定位影响SK‐N‐SH细胞的分化。NPM的表达及其在细胞分化过程中的分布值得进一步深入研究。j .细胞。生物化学学报，21(3):357 - 357,2010。©2010 Wiley‐Liss, Inc。
In this article, we selectively extracted the nuclear matrix and intermediate filament system of human neuroblastoma SK‐N‐SH cells pre‐ and post‐treated with retinoic acid (RA). The distribution of nucleophosmin (NPM) in the nuclear matrix and its colocalization with several products of related genes were investigated. Results from two‐dimensional gel electrophoresis and MALDI‐TOF showed that NPM was a component of the nuclear matrix and its expression in SK‐N‐SH cells post‐treated with RA was down‐regulated. Immunofluorescent microscopy observations further showed that NPM was localized in the nuclear matrix of SK‐N‐SH cells, and its expression level and distribution were altered after treatment with RA. The colocalization of NPM with c‐myc, c‐fos, p53, and Rb in SK‐N‐SH cells was observed under a laser scanning confocal microscope, but the colocalization region was changed by RA. Our results prove that NPM is a nuclear matrix protein, which is localized in nuclear matrix fibers. The colocalization of NPM with its related genes and oncogenes affect the differentiation of SK‐N‐SH cells. The expression of NPM and its distribution in the process of cell differentiation deserve more intensive investigation. J. Cell. Biochem. 111: 67–74, 2010. © 2010 Wiley‐Liss, Inc.