Study on Antimicrobial Activity of Extract of Prunella vulgaris L.

Study on Antimicrobial Activity of Extract of Prunella vulgaris L.
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发表时间:
2013
期刊:
Journal of Hainan Normal University
影响因子:
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通讯作者:
Jiang Guiyun
Jiang Guiyun
中科院分区:
其他
文献类型:
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作者:
Jiang Guiyun

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为研究夏枯草提取物的抑菌活性,采用溶剂回流提取法提取夏枯草有效成分,采用纸片扩散法观察不同溶剂提取物的抑菌活性,采用试管稀释法进行最低抑菌浓度实验。不同溶剂提取物的抑菌活性依次为乙酸乙酯、丁醇、乙醇、水,乙酸乙酯提取物对枯草芽孢杆菌的最低抑菌浓度(MIC)为2.5mg/ml,对金黄色葡萄球菌1.5mg/ml、大肠杆菌3.0mg/ml、匍匐根霉5.0mg/ml、曲霉10.0mg/ml无抑菌作用,对青霉无抑菌作用。
The antimicrobial activity of extracts of Prunella vulgaris L.was studied.The effective components from Prunella vulgaris L.were extracted using the solvent reflux extraction,The antimicrobial activity of different solvents extraction was observed through disk diffusion method,and minimum antibacterial concentrations experiments using the tube dilution method.The results showed,the different solvent extracts for the antibacterial activity were ethyl acetate butyl alcoholethanolwater.The minimum inhibitory concentration(MIC) of ethyl acetate extract were 2.5mg/ml for Bacillus subtil,1.5mg/ml for Staphylococcus aureus,3.0mg/ml for Escherichia coli,5.0mg/ml for Rhizopus stolonifer and 10.0mg/ml for Aspergillus.But it had no antibacterial effect on Penicillium.