A COMPARISON OF TRANSKETOLASE ASSAY AND TRANSKETOLASE AND LACTATE-DEHYDROGENASE ACTIVITY LEVELS IN WHOLE-BLOOD AND RED-CELL HEMOLYSATES AND IN LEUKOCYTES
A COMPARISON OF TRANSKETOLASE ASSAY AND TRANSKETOLASE AND LACTATE-DEHYDROGENASE ACTIVITY LEVELS IN WHOLE-BLOOD AND RED-CELL HEMOLYSATES AND IN LEUKOCYTES
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DOI：
10.1016/0305-0491(87)90397-x
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发表时间：
1987-01-01
影响因子：
2.2
通讯作者：
ABUFARSAK, F
中科院分区：
文献类型：
--
作者：
ALI, M;GUBLER, CJ;ABUFARSAK, F
1.研究了红细胞和白色细胞中的转酮醇酶活性，以及测定转酮醇酶活性和评估人和大鼠血液中“TPP效应”的条件。2.白色细胞中的转酮醇酶活性与红细胞中的转酮醇酶活性的比值在23和93之间变化。3.红细胞或白色细胞均可用于评估转酮醇酶活性和“TPP效应”，但用于评估转酮醇酶活性和添加二磷酸硫胺素后的百分比变化的最佳来源似乎是全血。
1. A study was made of transketolase activity in red and white blood cells and of conditions for assay for transketolase activity and for assessment of the "TPP effect" in human and rat blood. 2. The ratio of the transketolase activity in white cells to that in red cells varied between 23 and 93. 3. Red cells or white cells can both be used for assessment of transketolase activity and the "TPP effect", but the best source for evaluation of transketolase activity and the percent change in addition of thiamin diphosphate appears to be whole blood.