COMPLEMENTARY-DNA FOR A NOVEL HUMAN INTERLEUKIN (BSF-2) THAT INDUCES LYMPHOCYTES-B TO PRODUCE IMMUNOGLOBULIN
COMPLEMENTARY-DNA FOR A NOVEL HUMAN INTERLEUKIN (BSF-2) THAT INDUCES LYMPHOCYTES-B TO PRODUCE IMMUNOGLOBULIN
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DOI：
10.1038/324073a0
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发表时间：
1986-11-06
期刊：
影响因子：
64.8
通讯作者：
KISHIMOTO, T
中科院分区：
文献类型：
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作者：
HIRANO, T;YASUKAWA, K;KISHIMOTO, T
当受到抗原刺激时，B细胞受T细胞的影响增殖并分化为抗体形成细胞。自从有报道1,2可溶性因子可以在抗体反应中取代辅助性T细胞的某些功能以来，已经报道了几种不同种类的淋巴因子和单因子在b细胞的生长和分化中3,4。其中，人B细胞分化因子（BCDF或BSF-2）已被证明可诱导B细胞最终成熟为免疫球蛋白分泌细胞5 - 8。BSF-2纯化至均质9，并测定了其部分nh2末端氨基酸序列10。这些研究表明，BSF-2在功能和结构上与其他已知蛋白不同。本文报道了编码人BSF-2的cDNA的分子克隆、结构分析和功能表达。从cDNA中推断出BSF-2的初级序列，表明BSF-2是一种由184个氨基酸组成的新型白细胞介素。
When stimulated with antigen, B cells are influenced by T cells to proliferate and differentiate into antibody-forming cells. Since it was reported1,2that soluble factors could replace certain functions of helper T cells in the antibody response, several different kinds of lymphokines and monokines have been reported in B-cell growth and differentiation3,4. Among these, human B-cell differentiation factor (BCDF or BSF-2) has been shown to induce the final maturation of B cells into immunoglobulin-secreting cells5–8. BSF-2 was purified to homogeneity9and its partial NH2-terminal amino-acid sequence was determined10. These studies indicated that BSF-2 is functionally and structurally unlike other known proteins. Here, we report the molecular cloning, structural analysis and functional expression of the cDNA encoding human BSF-2. The primary sequence of BSF-2 deduced from the cDNA reveals that BSF-2 is a novel interleukin consisting of 184 amino acids.