Inflammation in cystic fibrosis airways: relationship to increased bacterial adherence
Inflammation in cystic fibrosis airways: relationship to increased bacterial adherence
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DOI：
10.1183/09031936.01.17100270
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发表时间：
2001-01-01
影响因子：
24.3
通讯作者：
Alton, EWFW
中科院分区：
文献类型：
--
作者：
Scheid, P;Kempster, L;Alton, EWFW
目前尚不清楚囊性纤维化（CF）肺的炎症是否主要与细菌感染有关，还是作为突变的囊性纤维化跨膜传导调节因子（CFTR）蛋白的直接结果，测量了CF和非CF细胞系以及CP和非CP人原代鼻上皮细胞的白细胞介素(IL)-8分泌，这些细胞与绿脓假单胞菌孵育或不孵育。通过凝胶移位法检测未受刺激的CF和非CF鼻上皮细胞、细胞系和小鼠组织中核因子- kb （NF-KB）的激活情况，在CF和非CF原代鼻细胞中，基础IL-8的产生和IVF-KB的激活没有显著差异
It is unclear whether inflammation in the cystic fibrosis (CF) lung relates predominantly to bacterial infection, or occurs as a direct consequence of mutant cystic fibrosis transmembrane conductance regulator (CFTR) protein,Interleukin (IL)-8 secretion from CF and non-CF cell lines, and from CP and non-CP human primary nasal epithelial cells incubated with or without Pseudomonas aevuginosa, was measured. Activation of nuclear factor-KB (NF-KB) in unstimulated CF and non-CF nasal epithelial cells, cell lines and murine tissues was measured by gel-shift assays,No significant difference in basal IL-8 production or IVF-KB activation was observed between CF and non-CF primary nasal cells, However, CF cells exhibited a significantly (p