Single-Cell Isolation and Gene Analysis: Pitfalls and Possibilities.
Single-Cell Isolation and Gene Analysis: Pitfalls and Possibilities.
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DOI：
10.3390/ijms161125996
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发表时间：
2015-11-10
影响因子：
5.6
通讯作者：
Weltzien FA
中科院分区：
文献类型：
--
作者：
Hodne K;Weltzien FA
在过去的二十年中，单细胞分析（SCA）揭示了广泛的表型差异在同质细胞群体。这些表型差异反映在基因调控的随机性中，这通常被整个组织分析中的定性和定量平均所掩盖。分离转录本和研究基因如何在单细胞水平上被调节的能力需要高度敏感和精细的方法。本文综述了目前用于SCA的不同策略，包括RNA的收获、逆转录和扩增，以及转录物定量的方法。这篇综述提供了SCA的历史背景，讨论了这个令人兴奋的研究领域的局限性以及当前和未来的可能性。
During the last two decades single-cell analysis (SCA) has revealed extensive phenotypic differences within homogenous cell populations. These phenotypic differences are reflected in the stochastic nature of gene regulation, which is often masked by qualitatively and quantitatively averaging in whole tissue analyses. The ability to isolate transcripts and investigate how genes are regulated at the single cell level requires highly sensitive and refined methods. This paper reviews different strategies currently used for SCA, including harvesting, reverse transcription, and amplification of the RNA, followed by methods for transcript quantification. The review provides the historical background to SCA, discusses limitations, and current and future possibilities in this exciting field of research.