Transfer of Thiolated DNA Staples from DNA Origami Nanostructures to Self-Assembled Monolayer-Passivated Gold Surfaces: Implications for Interfacial Molecular Recognition
Transfer of Thiolated DNA Staples from DNA Origami Nanostructures to Self-Assembled Monolayer-Passivated Gold Surfaces: Implications for Interfacial Molecular Recognition
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DOI：
10.1021/acsanm.1c01685
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发表时间：
2021-07
影响因子：
5.9
通讯作者：
Qufei Gu;Yehan Zhang;Huan H. Cao;Songtao Ye;T. Ye
中科院分区：
文献类型：
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作者：
Qufei Gu;Yehan Zhang;Huan H. Cao;Songtao Ye;T. Ye
我们表明，DNA折纸纳米结构上显示的硫醇链可以转移到金表面时，DNA折纸瓦沉积到自组装单层（SAM）钝化表面。空间统计分析显示，油墨分子转移产率为70%，与现有的基于DNA纳米结构的纳米压印方法实现的最高产率相当。表面钝化减少非特异性吸附，并允许高分辨率，无标记的表征转移的空间模式。因此，我们的方法提供了一种形成复杂的单分子纳米阵列的途径，用于阐明酶级联和界面分子识别的结构-功能关系。
We show that thiolated strands displayed on a DNA origami nanostructure can be transferred to a gold surface when the DNA origami tile is deposited onto a self-assembled monolayer (SAM) passivating the surface. Spatial statistical analysis revealed the ink molecule transfer yield to be 70%, comparable to the highest yield achieved with existing DNA-nanostructure-based nanoimprinting methods. The surface passivation reduces nonspecific adsorption and allows high-resolution, label-free characterization of the transferred spatial patterns. Therefore, our method offers a pathway toward forming complex single-molecule nanoarrays for elucidating the structure–function relationships of enzyme cascades and interfacial molecular recognition.