A set of inhibitors for discrimination between the basic isozymes of glutathione transferase in rat liver.
A set of inhibitors for discrimination between the basic isozymes of glutathione transferase in rat liver.
复制标题
一组用于区分大鼠肝脏中谷胱甘肽转移酶基本同工酶的抑制剂。
DOI：
10.1016/0006-291x(83)90856-2
复制
发表时间：
1983
影响因子：
3.1
通讯作者：
B. Mannervik
中科院分区：
文献类型：
--
作者：
S. Yalcin;H. Jensson;B. Mannervik
一组抑制剂，包括血红素，溴磺酞，溴化三乙基锡用于区分和鉴定的主要碱性同工酶的谷胱甘肽转移酶在大鼠肝细胞质。六种酶由4种蛋白质亚基A、B、C和L的二元组合形成。转移酶之间的区分可以基于亚基对抑制剂的敏感性的差异。转移酶亚基的鉴定还得到了特异性底物和抑制剂联合使用的支持。
A set of inhibitors including hematin, bromosulfophthalein, and triethyltin bromide was used for discrimination and identification of the major basic isozymes of glutathione transferase in rat liver cytosol. Six enzymes are formed as binary combinations of 4 protein subunits: A, B, C, and L. Discrimination between the transferases can be based on the differences of the subunits in susceptibility to the inhibitors. The identification of transferase subunits is further supported by the combined use of specific substrates and inhibitors.