Association of host cell endoplasmic reticulum and mitochondria with the Toxoplasma gondii parasitophorous vacuole membrane: a high affinity interaction.
Association of host cell endoplasmic reticulum and mitochondria with the Toxoplasma gondii parasitophorous vacuole membrane: a high affinity interaction.
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发表时间：
1997-09
影响因子：
4
通讯作者：
A. Sinai;P. Webster;K. Joiner
中科院分区：
文献类型：
--
作者：
A. Sinai;P. Webster;K. Joiner
专性细胞内原生动物弓形虫的寄生液泡膜（PVM）与宿主线粒体和内质网（ER）紧密相关。我们使用形态计量学和生化方法的组合来表征这种独特的现象，我们称之为pvm -细胞器关联。PVM与相关线粒体和内质网的平均距离分别为12 nm和18 nm。pvm -细胞器关联的建立依赖于活跃的寄生虫进入，但不需要寄生虫的生存能力来维持。结合力不是施加在生长液泡上的空间限制的结果。形态计量学分析表明，随着液泡的增大，线粒体与PVM的关联程度保持不变，而ER的关联程度则降低。破坏宿主细胞微管可部分阻断pvm -线粒体关联的建立，但不能维持PVM-ER关联，对PVM-ER关联无显著影响。通过电子显微镜和亚细胞分离评估，PVM-细胞器关联在被感染宿主细胞破坏后仍保持不变，显示固定的PVM和细胞器标记物共同迁移。综上所述，这些数据表明，寄生虫和细胞器成分之间的高亲和力，潜在的蛋白质-蛋白质相互作用是pvm -细胞器结合的原因。
The parasitophorous vacuole membrane (PVM) of the obligate intracellular protozoan parasite Toxoplasma gondii forms tight associations with host mitochondria and the endoplasmic reticulum (ER). We have used a combination of morphometric and biochemical approaches to characterize this unique phenomenon, which we term PVM-organelle association. The PVM is separated from associated mitochondria and ER by a mean distance of 12 and 18 nm, respectively. The establishment of PVM-organelle association is dependent on active parasite entry, but does not require parasite viability for its maintenance. Association is not a consequence of spatial constraints imposed on the growing vacuole. Morphometric analysis indicates that the extent of mitochondrial association with the PVM stays constant as the vacuole enlarges, whereas the extent of ER association decreases. Disruption of host cell microtubules partially blocks the establishment but not the maintenance of PVM-mitochondrial association, and has no significant effect on PVM-ER association. PVM-organelle association is maintained following disruption of infected host cells, as assessed by electron microscopy and by sub-cellular fractionation showing co-migration of fixed PVM and organelle markers. Taken together, the data suggest that a high affinity, potentially protein-protein interaction between parasite and organelle components is responsible for PVM-organelle association.