Evidence of a high proportion of premature unbalanced separation of sister chromatids in the first polar bodies of women of advanced age
Evidence of a high proportion of premature unbalanced separation of sister chromatids in the first polar bodies of women of advanced age
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DOI：
10.1093/humrep/dei484
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发表时间：
2006-05-01
影响因子：
6.1
通讯作者：
Selva, J
中科院分区：
文献类型：
--
作者：
Vialard, F;Petit, C;Selva, J
背景：母亲衰老是与非整倍体明确相关的唯一病因因素。两种机制似乎可以解释卵母细胞的这些异常：姐妹染色单体的不分离和过早不平衡分离（PSSC）。先前对未受精卵母细胞的研究认为PSSC在老年妇女非整倍体的病因学中起主要作用，但在体外，卵母细胞的老化可能会影响结果。方法：由于高龄妇女非整倍体的高患病率，在辅助生殖计划中，在向38岁以上妇女提供ICSI之前对第一极体进行染色体筛查。结果：29例女性（平均年龄40岁2个月）141个卵母细胞中，异常43个（30.5%）。发现65例异常，80%的病例涉及PSSC。结论：这些结果与以往的研究一致，并证实了PSSC在“新鲜”卵母细胞中在老年妇女非整倍体的病因中起主要作用。
BACKGROUND: Maternal ageing is the only aetiological factor unequivocally linked to aneuploidy. Two mechanisms seem to explain these abnormalities in oocytes: non-disjunction and premature unbalanced separation of sister chromatids (PSSC). Previous studies of unfertilized oocytes argue for a major role of PSSC in the aetiology of aneuploidy for women of advanced age, but in vitro ageing of the oocytes could influence the results. METHODS: Owing to the high prevalence of aneuploidy in women of advanced age, chromosomal screening of the first polar body just before ICSI was offered to women (from 38 years of age) included in an assisted reproduction programme. RESULTS: Among 141 oocytes from 29 women (mean age 40 years and 2 months), 43 (30.5%) were abnormal. Sixty-five abnormalities were found and PSSC was involved in 80% of cases. CONCLUSION: These results are in accordance with previous studies and confirm, in 'fresh' oocytes, the major role of PSSC in the aetiology of aneuploidy in women of advanced age.