Expression levels of vascular endothelial growth factor, matrix metalloproteinases 2 and 9 and tissue inhibitor of metalloproteinases 1 and 2 in the plasma of patients with ovarian carcinoma
Expression levels of vascular endothelial growth factor, matrix metalloproteinases 2 and 9 and tissue inhibitor of metalloproteinases 1 and 2 in the plasma of patients with ovarian carcinoma
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DOI：
10.1016/s0959-8049(03)00427-1
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发表时间：
2003-09-01
影响因子：
8.4
通讯作者：
Giavazzi, R
中科院分区：
文献类型：
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作者：
Manenti, L;Paganoni, P;Giavazzi, R
我们测量了卵巢癌患者 (n = 40)、其他妇科病变 (n = 30) 和子宫内膜癌患者血浆中血管内皮生长因子 (VEGF)、2 型和 9 型基质金属蛋白酶（MMP-2 和 MMP-9）以及基质金属蛋白酶 1 和 2 组织抑制剂（TIMP-1 和 TIMP-2）的水平。 健康志愿者的血浆（n = 26）。通过酶谱法测量 MMP-2 和 MMP-9（原形式和活性形式）明胶分解活性。使用酶联免疫吸附测定 (ELISA) 测定可溶性 VEGF 和 TIMP。卵巢癌患者术前血浆 VEGF 水平显着高于健康志愿者（P
We measured the levels of the vascular endothelial growth factor (VEGF), matrix metalloproteinases type 2 and type 9 (MMP-2 and MMP-9) and tissue inhibitors of matrix metalloproteinase 1 and 2 (TIMP-1 and TIMP-2) in the plasma of patients with ovarian carcinoma (n = 40), in other gynaecological pathologies (n = 30) and in the plasma of healthy volunteers (n = 26). MMP-2 and MMP-9 (pro and active forms) gelatinolytic activity was measured by zymography. Enzyme-linked immunosorbent assays (ELISA) were used to assay soluble VEGF and TIMPs. Preoperative plasma VEGF levels were significantly higher in patients with ovarian cancer than in healthy volunteers (P