Interactions of the c-di-GMP riboswitch with its second messenger ligand.
Interactions of the c-di-GMP riboswitch with its second messenger ligand.
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DOI：
10.1042/bst0390647
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发表时间：
2011-04
影响因子：
3.9
通讯作者：
Strobel SA
中科院分区：
文献类型：
--
作者：
Smith KD;Strobel SA
C-di-GMP核糖开关是c-di-GMP第二信使信号通路中的一个大分子靶点。它调控许多与c-di-GMP代谢相关的基因，以及参与细菌运动、毒力和生物被膜形成的基因。核糖开关与这种对称二核苷酸的碱基和磷酸骨架进行不对称接触。系统发育学和诱变学已经证实，这是一个灵活的基序，其中变体可以与c-di-GMP的每个鸟嘌呤碱基进行选择性相互作用。已经设计了一种突变的核糖开关，可以与相关分子c-di-AMP结合，确认与配体建立的最重要的接触。结合动力学表明，这是一种受动力学控制的核糖开关，核糖开关的突变会导致脱落率的增加。因此，这种核糖开关在序列和动力学性质上是灵活的。
The c-di-GMP riboswitch is a macromolecular target in the c-di-GMP second messenger signaling pathway. It regulates many genes related to c-di-GMP metabolism as well as genes involved in bacterial motility, virulence and biofilm formation. The riboswitch makes asymmetric contacts to the bases and phosphate backbone of this symmetric dinucleotide. The phylogenetics suggested and mutagenesis has confirmed that this is a flexible motif where variants can make alternative interactions with each of the guanine bases of c-di-GMP. A mutant riboswitch has been designed that can bind a related molecule, c-di-AMP, confirming the most important contacts made to the ligand. The binding kinetics reveal that this is a kinetically controlled riboswitch and mutations to the riboswitch lead to increases in the off-rate. This riboswitch is therefore flexible in sequence as well as kinetic properties.