Glycoprotein tyrosine phosphorylation in Rous sarcoma virus-transformed chicken embryo fibroblasts.
Glycoprotein tyrosine phosphorylation in Rous sarcoma virus-transformed chicken embryo fibroblasts.
复制标题
劳斯肉瘤病毒转化的鸡胚成纤维细胞中糖蛋白酪氨酸磷酸化。
DOI：
10.1128/mcb.10.2.837-841.1990
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发表时间：
1990
影响因子：
5.3
通讯作者：
Weber,MJ
中科院分区：
文献类型：
--
作者：
Kozma,LM;Reynolds,AB;Weber,MJ
为了鉴定磷酸化与表型转化相关的细胞膜蛋白，研究了用小麦胚芽凝集素色谱法分离的多种激酶阳性/转化缺陷突变体感染的细胞中细胞糖蛋白的酪氨酸磷酸化水平。我们鉴定了两种糖蛋白，分子质量分别为95和135千道尔顿，其磷酸化与形态转化、软琼脂生长和2-脱氧葡萄糖摄取速率的增加有关。这些蛋白的转化与磷酸化之间的强相关性表明它们可能是pp60src的底物，这在转化过程中是重要的。
The level of tyrosine phosphorylation of cellular glycoproteins isolated by wheat germ agglutinin chromatography in cells infected with a variety of kinase-positive/transformation-defectivesrcmutants was examined in an effort to identify cellular membrane proteins whose phosphorylation correlates with phenotypic transformation. We have identified two glycoproteins, with molecular masses of 95 and 135 kilodaltons, whose phosphorylation correlates with morphological transformation, growth in soft agar, and an increase in the rate of 2-deoxyglucose uptake. The strong correlation obtained between transformation and phosphorylation of these proteins suggests that they may be substrates for pp60srcwhich are important in the process of transformation.