SEPARATION OF SYNTROPHOMONAS-WOLFEI FROM METHANOSPIRILLUM-HUNGATII IN SYNTROPHIC COCULTURES BY USING PERCOLL GRADIENTS
SEPARATION OF SYNTROPHOMONAS-WOLFEI FROM METHANOSPIRILLUM-HUNGATII IN SYNTROPHIC COCULTURES BY USING PERCOLL GRADIENTS
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DOI：
10.1128/aem.53.5.1183-1185.1987
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发表时间：
1987-05-01
影响因子：
4.4
通讯作者：
MCINERNEY, MJ
中科院分区：
文献类型：
--
作者：
BEATY, PS;WOFFORD, NQ;MCINERNEY, MJ
Percoll梯度离心可有效地分离沃氏互养单胞菌细胞和亨氏甲烷菌细胞，导致S. wolfei细胞相对M. Hungatii细胞分离的S. Wolfei细胞是活的。获得了克数量的细胞蛋白质，其具有酶活性并且具有低水平的产甲烷辅因子factor420的污染。
Percoll gradient centrifugation effectively separated Syntrophomonas wolfei cells from Methanospirillum hungatii cells, resulting in a 70- to 80-fold enrichment of S. wolfei cells relative to M. hungatii cells. The separated S. wolfei cells were viable. Gram quantities of cellular protein which was enzymatically active and had low levels of contamination by the methanogenic cofactor, factor420, were obtained.