Domoic acid production by Pseudo-nitzschia calliantha Lundholm, Moestrup et Hasle (bacillariophyta) isolated from the Black Sea
Domoic acid production by Pseudo-nitzschia calliantha Lundholm, Moestrup et Hasle (bacillariophyta) isolated from the Black Sea
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DOI：
10.1016/j.hal.2007.09.004
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发表时间：
2008-06-01
期刊：
影响因子：
6.6
通讯作者：
Lee, Raisa
中科院分区：
文献类型：
--
作者：
Besiktepe, Sengul;Ryabushko, Larisa;Lee, Raisa
从黑海塞瓦斯托波尔湾分离出一种伪尼茨氏虫，对其毒性进行了研究。经扫描电镜和透射电镜鉴定为P. calliantha Lundholm, Moestrup et Hasle。在花莲的整个生长周期中，分批培养均检测到软骨藻酸（DA）。通过氟酰甲氧基羰基（FMOC）衍生化和hplc -荧光法证实了该硅藻产DA。细胞DA水平在指数期早期较高，最大值为0.95 pg（-1）。在固定期，细胞内DA水平下降。这是从黑海分离出的第一个生产硅藻的DA记录。(C) 2007 Elsevier B.V.版权所有
A species of Pseudo-nitzschia isolated from Sevastopol Bay, Black Sea, was examined for its toxicity. The species was identified as P. calliantha Lundholm, Moestrup et Hasle based on SEM and TEM examination. Domoic acid (DA) was detected in batch culture throughout the growth cycle of P. calliantha. The production of DA by this diatom species was confirmed by fluorenylmethoxycarbonyl (FMOC) derivatization and HPLC-fluorescence method. The cellular DA level was higher in the early exponential phase, with the maximum value of 0.95 pg DA cell(-1). In the stationary phase, the cellular DA levels declined. This is the first record of a DA producing diatom isolated from the Black Sea. (C) 2007 Elsevier B.V. All rights reserved.