The role of hydroperoxides as calcium release agents in rat brain mitochondria.
The role of hydroperoxides as calcium release agents in rat brain mitochondria.
复制标题
氢过氧化物作为大鼠脑线粒体钙释放剂的作用。
DOI：
10.1016/0003-9861(84)90501-0
复制
发表时间：
1984
影响因子：
3.9
通讯作者：
C. Richter
中科院分区：
文献类型：
--
作者：
J. Satrústegui;C. Richter
先前已显示氢过氧化物通过特定的释放途径诱导完整大鼠肝线粒体中的Ca 2+释放。在这里，据报道，在大鼠脑线粒体中，过氧化氢诱导的Ca 2+释放也是可操作的，但不太重要。过氧化氢刺激钙2+释放钌红的存在下，在约两倍的Ca 2+负载的40 nmol/mg线粒体蛋白。加入过氧化氢后，Na+仍能诱发脑线粒体Ca ~（2+）释放。在存在琥珀酸和鱼藤酮的情况下，氢过氧化物仅诱导非常有限的吡啶核苷酸氧化，最可能是由于脑线粒体中发现的谷胱甘肽过氧化物酶（EC 1.11.1.9）和谷胱甘肽还原酶（EC 1.6.4.2）水平低。与肝线粒体类似，在脑线粒体中发现NAD酶（EC3.2.2.5）活性。然而，它对ADP和ATP的定位和敏感性与肝线粒体酶不同。
Hydroperoxides have previously been shown to induce Ca2+release from intact rat liver mitochondria via a specific release pathway. Here it is reported that, in rat brain mitochondria, a hydroperoxide-induced Ca2+release is also operative but is of minor importance. Hydroperoxide stimulates Ca2+release in the presence of ruthenium red about twofold at a Ca2+load of 40 nmol/mg mitochondrial protein. After addition of hydroperoxide, Ca2+release from brain mitochondria can still be evoked by Na+. In the presence of succinate and rotenone, hydroperoxide induces only a very limited oxidation of pyridine nucleotides, most probably due to the low level of glutathione peroxidase (EC 1.11.1.9) and glutathione reductase (EC 1.6.4.2) found in brain mitochondria. Similar to liver mitochondria, a NADase (EC 3.2.2.5) activity is found in brain mitochondria. Its localization and sensitivity toward ADP and ATP, however, is different from that of the liver mitochondrial enzyme.