Cytokines and visceral leishmaniasis: a comparison of plasma cytokine profiles between the clinical forms of visceral leishmaniasis
Cytokines and visceral leishmaniasis: a comparison of plasma cytokine profiles between the clinical forms of visceral leishmaniasis
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DOI：
10.1590/s0074-02762012000600005
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发表时间：
2012-09-01
期刊：
影响因子：
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通讯作者：
Caldas, Arlene de Jesus Mendes
中科院分区：
文献类型：
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作者：
Costa, Alinne Silva Andrade;Costa, Graciomar Conceição;Caldas, Arlene de Jesus Mendes
目前还没有很好地确定是否细胞因子的生产不同的反应，不同的临床形式的内脏利什曼病（VL）。在这项工作中，我们进行了一项横断面研究，以调查参与VL发病机制的细胞因子[干扰素（IFN）-γ，肿瘤坏死因子（TNF）-α，白细胞介素（IL）-2，IL-4，IL-10和IL-12]在80名受试者中，包括活动性VL受试者，无症状感染者，治愈VL受试者和未感染的对照组。这些患者是通过从转诊医院抽样和从基于人群的队列研究中随机选择来招募的。结果显示，各组间血浆中所有细胞因子浓度均存在显著性差异（p < 0.05）。活动性疾病患者的IL-10、IL-4、INF-γ和TNF-α的血浆水平高于其他组，并且他们产生的IL-12高于无症状和治愈的受试者。只有无症状和治愈的受试者的IL-2浓度高于活动性疾病患者（p < 0.05）。我们的结果表明，这些细胞因子可用作流行病学研究中的标记物，以区分不同临床形式的VL。然而，它们的有用性应在其他流行地区进行的调查中得到证实。
It is not well established whether cytokine production differs in response to different clinical forms of visceral leishmaniasis (VL). In this work, we performed a cross-sectional study to investigate the plasma levels of cytokines [interferon (IFN)-gamma, tumour necrosis factor (TNF)-alpha, interleukin (IL)-2, IL-4, IL-10 and IL-12] involved in the pathogenesis of VL in 80 subjects from VL endemic areas, including subjects with active VL, subjects with asymptomatic infection, subjects with cured VL and uninfected controls. The patients were recruited by sampling from a referral hospital and by random selection from a population-based cohort study. The results showed significant differences in the plasma concentration of all cytokines between the groups (p < 0.05). Patients with the active disease had higher plasma levels of IL-10, IL-4, INF-gamma and TNF-alpha relative to the other groups and they produced more IL-12 than asymptomatic and cured subjects. Only the IL-2 concentration was higher in the asymptomatic and cured subjects relative to the patients with active disease (p < 0.05). Our results suggest that these cytokines can be used as markers in epidemiological studies conducted in endemic areas to distinguish between different clinical forms of VL. However, their usefulness should be confirmed in investigations conducted in other endemic areas.