Putative regulatory mechanism of prothoracicotropic hormone (PTTH) secretion in the American cockroach, Periplaneta americana as inferred from co-localization of Rab8, PTTH, and protein kinase C in neurosecretory cells
Putative regulatory mechanism of prothoracicotropic hormone (PTTH) secretion in the American cockroach, Periplaneta americana as inferred from co-localization of Rab8, PTTH, and protein kinase C in neurosecretory cells
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DOI：
10.1007/s00441-008-0747-9
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发表时间：
2009-03-01
影响因子：
3.6
通讯作者：
Takeda, Makio
中科院分区：
文献类型：
--
作者：
Hiragaki, Susumu;Uno, Tomohide;Takeda, Makio
Rab家族的小GTP酶作为囊泡运输途径的重要调节剂，包括神经激素的胞吐作用。这些过程在昆虫中还没有被很好地理解。为了解决Rab蛋白的生理功能和它们在昆虫神经分泌中的磷酸化，Rab 8样，促前胸腺激素（PTTH）样，蛋白激酶C（PKC）样免疫组织化学反应（-IR）在美洲大蠊，美洲大蠊脑中进行了研究。所有测试的抗体与大脑间部、心体和咽侧体神经中的神经元反应。双标记实验表明，所有PTTH-IR共定位与Rab 8-IR和PKC-IR在大脑间部，虽然排他性的反应是目前对Rab 8或PKC的抗血清。这些发现支持Rab 8样抗原被PKC磷酸化的观点，并且这种磷酸化参与了该物种PTTH的轴突运输和分泌。
Small GTPases of the Rab family act as essential regulators of vesicle transport pathways, including the exocytosis of neurohormones. These processes are not well-understood in insects. To address the physiological function of Rab proteins and their phosphorylation in insect neurosecretion, Rab8-like, prothoracicotropic hormone (PTTH)-like, and protein kinase C (PKC)-like immunohistochemical reactivities (-ir) were investigated in the brain of the American cockroach, Periplaneta americana. All the antibodies tested reacted with neurons in the pars intercerebralis, corpora cardiaca, and nervi corporis allati I. Double-labeling experiments demonstrated that all PTTH-ir were colocalized with Rab8-ir and PKC-ir in the pars intercerebralis, although exclusive reactivity was present to antisera against Rab8 or PKC. These findings support the notion that Rab8-like antigen is phosphorylated by PKC, and that this phosphorylation is involved in the axonal transport and secretion of PTTH in this species.