PTC1 Is Required for Vacuole Inheritance and Promotes the Association of the Myosin-V Vacuole-specific Receptor Complex
PTC1 Is Required for Vacuole Inheritance and Promotes the Association of the Myosin-V Vacuole-specific Receptor Complex
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DOI：
10.1091/mbc.e08-09-0954
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发表时间：
2009-03-01
影响因子：
3.3
通讯作者：
Weisman, Lois S.
中科院分区：
文献类型：
--
作者：
Jin, Yui;Eves, P. Taylor;Weisman, Lois S.
细胞器遗传发生在细胞分裂期间。在酿酒酵母中，液泡的遗传、线粒体和皮层内质网的分布受Ptc 1 p（一种2C型蛋白磷酸酶）的调节。在这里，我们表明，PTC 1/VAC 10控制分布的额外的货物移动肌球蛋白V电机。这些包括过氧化物酶体、分泌囊泡、Myo 2 p的货物和Myo 4p的货物ASH 1 mRNA。我们发现Ptc 1 p是Myo 2 p和Myo 4p正确分布所必需的。令人惊讶的是，PTC 1也需要维持细胞器特异性受体的稳态水平，包括Vac 17 p，Inp 2 p和Mmr 1 p，它们分别将Myo 2 p连接到液泡，过氧化物酶体和线粒体。此外，Vac 17 p融合到Myo 2 p的货物结合结构域抑制ptc 1 Delta细胞中的空泡遗传缺陷。这些发现表明，PTC 1促进肌球蛋白V与其细胞器特异性衔接蛋白的关联。此外，这些观察结果表明，尽管存在细胞器特异性受体，但存在协调不同细胞成分运动的高阶调节。
Organelle inheritance occurs during cell division. In Saccharomyces cerevisiae, inheritance of the vacuole, and the distribution of mitochondria and cortical endoplasmic reticulum are regulated by Ptc1p, a type 2C protein phosphatase. Here we show that PTC1/VAC10 controls the distribution of additional cargoes moved by a myosin-V motor. These include peroxisomes, secretory vesicles, cargoes of Myo2p, and ASH1 mRNA, a cargo of Myo4p. We find that Ptc1p is required for the proper distribution of both Myo2p and Myo4p. Surprisingly, PTC1 is also required to maintain the steady-state levels of organelle-specific receptors, including Vac17p, Inp2p, and Mmr1p, which attach Myo2p to the vacuole, peroxisomes, and mitochondria, respectively. Furthermore, Vac17p fused to the cargo-binding domain of Myo2p suppressed the vacuole inheritance defect in ptc1 Delta cells. These findings suggest that PTC1 promotes the association of myosin-V with its organelle-specific adaptor proteins. Moreover, these observations suggest that despite the existence of organelle-specific receptors, there is a higher order regulation that coordinates the movement of diverse cellular components.