Alveolar macrophages, blood monocytes, and density-fractionated alveolar macrophages differ in their ability to promote lymphocyte proliferation to mitogen and antigen.
Alveolar macrophages, blood monocytes, and density-fractionated alveolar macrophages differ in their ability to promote lymphocyte proliferation to mitogen and antigen.
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肺泡巨噬细胞、血单核细胞和密度分级的肺泡巨噬细胞促进淋巴细胞增殖至丝裂原和抗原的能力不同。
DOI：
10.1164/arrd.1987.135.3.682
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Rossman,MD
中科院分区：
文献类型：
--
作者：
Ferro,TJ;Kern,JA;Elias,JA;Kamoun,M;Daniele,RP;Rossman,MD
我们比较了正常志愿者的肺泡巨噬细胞(AM)、单核细胞(BM)和密度分离的AM支持抗原和有丝分裂原诱导的自体T细胞增殖的相对能力。AM能够促进T细胞对抗原的增殖反应，但效果不如BM。密度分级的AM支持T细胞反应的能力是不同的。密度最高的AM比密度最低的AM增殖更多与抗原诱导的反应相比，AM和密度分离的AM比BM更有效地支持丝裂原诱导的反应。AM、密度分级AM和BM支持T细胞反应的能力与II类主要组织相容性决定因素的表面表达无关。当使用低密度AM时，加入纯化的IL-1可以部分恢复T细胞的增殖，而当使用普通AM时，则不能促进T细胞的增殖。这表明，IL-1活性降低可能是低密度AM促进T细胞反应能力降低的部分原因，但其他过程也可能是AM、BM和密度分离AM之间辅助细胞功能差异的原因
We compared the relative abilities of alveolar macrophages (AM), blood monocytes (BM), and density-fractionated AM to support antigen- and mitogen-induced proliferation of autologous T cells in normal volunteers. The AM were able to promote the T-cell proliferative response to antigen, but they did so less effectively than did the BM. Density-fractionated AM were heterogeneous in their ability to support T-cell responses. More proliferation occurred with the densest AM than with the least dense AM. In contrast to antigen-induced responses, mitogen-induced responses were supported more effectively by AM and density-fractionated AM than by BM. The ability of AM, density-fractionated AM, and BM to support T-cell responses did not correlate with surface expression of class II major histocompatibility determinants. Addition of purified IL-1 resulted in a partial restoration of T-cell proliferation when low-density AM were used but no augmentation when unfractionated AM were used. This suggests that reduced IL-1 activity may partially explain the decreased ability of low density AM to promote T-cell responses, but that other processes may also contribute to differences in accessory cell function among AM, BM, and density-fractionated AM.