Generation of pluripotent stem cells from neonatal mouse testis
Generation of pluripotent stem cells from neonatal mouse testis
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DOI：
10.1016/j.cell.2004.11.011
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发表时间：
2004-12-29
期刊：
影响因子：
64.5
通讯作者：
Shinohara, T
中科院分区：
文献类型：
--
作者：
Kanatsu-Shinohara, M;Inoue, K;Shinohara, T
尽管生殖系细胞可以形成多能胚胎干（ES）/胚胎生殖（EG）细胞，但这些细胞只能来源于胚胎组织，并且这样的多能细胞还不能从新生儿性腺获得。我们成功地从新生小鼠睾丸中建立了ES样细胞。这些ES样细胞的表型相似的ES/ EG细胞，除了在他们的基因组印迹模式。它们在用于诱导ES细胞分化的条件下在体外分化成各种类型的体细胞，并在接种到小鼠体内后产生畸胎瘤。此外，这些ES样细胞在注入囊胚时形成生殖系嵌合体。因此，新生儿睾丸中存在形成多能细胞的能力。从新生儿睾丸中获得多能干细胞的能力对生殖细胞生物学具有重要意义，并为将这些细胞用于生物技术和医学开辟了可能性。
Although germline cells can form multipotential embryonic stem (ES)/embryonic germ (EG) cells, these cells can be derived only from embryonic tissues, and such multipotent cells have not been available from neonatal gonads. Here we report the successful establishment of ES-like cells from neonatal mouse testis. These ES-like cells were phenotypically similar to ES/ EG cells except in their genomic imprinting pattern. They differentiated into various types of somatic cells in vitro under conditions used to induce the differentiation of ES cells and produced teratomas after inoculation into mice. Furthermore, these ES-like cells formed germline chimeras when injected into blastocysts. Thus, the capacity to form multipotent cells persists in neonatal testis. The ability to derive multipotential stem cells from the neonatal testis has important implications for germ cell biology and opens the possibility of using these cells for biotechnology and medicine.