Strengths and limitations of immunoassays for effective and efficient use for pesticide analysis in water samples: A review
Strengths and limitations of immunoassays for effective and efficient use for pesticide analysis in water samples: A review
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DOI：
10.1016/s0003-2670(97)00608-9
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发表时间：
1998-04-24
影响因子：
6.2
通讯作者：
Barcelo, D
中科院分区：
文献类型：
--
作者：
Hennion, MC;Barcelo, D
免疫分析技术为农药分析提供了一种简单、有效和廉价的方法。然而，与更传统的技术相比，免疫测定法的接受取决于其质量和有效性的证明。本文主要介绍免疫测定方法的基本知识，以便更好地利用免疫测定方法，特别是酶联免疫吸附试验。特别注意更好地理解某些免疫测定法获得的高选择性和敏感性，而其他免疫测定法则没有。还解释了为什么一些免疫测定是定量方法，而另一些只能用作筛选方法。交叉反应过程、样品矩阵的影响和数据解释通过大量的例子从文献中说明。其他格式，特别是流动注射免疫分析，试纸免疫分析和脂质体扩增免疫分析提出。给出了验证和使用的质量保证和指南。(C) 1998爱思唯尔科学有限公司
Immunoassay techniques provide a simple, powerful and inexpensive method for pesticide analysis. However, the acceptance of immunoassays is dependent on the demonstration of quality and validity compared to more traditional techniques. In this review, primarily, the knowledge and the fundamentals of immunoassay methods are given in order to make good use of immunoassays, especially of ELISA tests. Special attention is given to a better understanding of the high selectivity and sensitivity which is attained for some immunoassays and not for others. It is also explained why some immunoassays are a quantitative method whereas others can only be used as a screening method. The cross-reactivity process, the effect of the sample matrix and the data interpretation are illustrated oy numerous examples from the literature. Other formats, especially flow-injection immunoassays, dipstick immunoassay and liposome-amplified immunoassays are presented. Quality assurance and guidelines for validation and use are given. (C) 1998 Elsevier Science B.V.