Quantitative expression of RIG-like helicase, NOD-like receptor and inflammasome-related mRNAs in humans and mice
Quantitative expression of RIG-like helicase, NOD-like receptor and inflammasome-related mRNAs in humans and mice
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DOI：
10.1093/intimm/dxq058
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发表时间：
2010-09-01
影响因子：
4.4
通讯作者：
Anders, Hans-Joachim
中科院分区：
文献类型：
--
作者：
Lech, Maciej;Avila-Ferrufino, Alejandro;Anders, Hans-Joachim
表面和内体定位的 Toll 样受体的细胞类型、器官和物种特异性表达已得到很好的描述，但对胞质模式识别分子各自的表达谱知之甚少。因此，我们测定了人和小鼠 11 个实体器官中 15 种胞质模式识别分子的 mRNA 表达水平。人体器官显示大多数分子的 mRNA 水平与脾脏中的水平较低，但脑中炎症体相关的 NOD、富含亮氨酸重复序列和含热蛋白结构域的蛋白 1-3 (NLRP1-3) 和 -12、肝脏中的 LGP2、视黄酸诱导基因 I (RIG-I) 和 NLRP10、小肠中的 NLRP10、LGP2、RIG-I、NAIP、睾丸中的 NLRP2 和 -3 以及肌肉中的 RIG-I、NLRP2 和 -10。在小鼠中，与脾脏相比，大多数器官的 mRNA 表达水平也较低。仅肝脏中的 NLRP6、小肠中的 NAIP 和 NLRP6、结肠中的 LGP2、核苷酸结合寡聚化结构域 1 (NOD1)、NLRP1、-2、-6、-10 和 -12 以及肾脏中的 MDA5、RIG-I、NLRC4、NOD1、-2、NLRP1、-2、-6、-10 和 -12 mRNA 水平较高。静息的人和小鼠单核细胞和 T 细胞表达大多数分子，并在激活后产生 IL-1 β 和 CCL5/RANTES。然而，鼠单核细胞在激活后强烈上调受体表达，而人单核细胞下调受体表达。这些数据表明，在相关研究的设计和解释中需要考虑细胞类型、器官和物种特异性的表达和调节。
The cell-type-, organ- and species-specific expression of the surface and endosomally located Toll-like receptors are well described but little is known about the respective expression profiles of cytosolic pattern recognition molecules. We therefore determined the mRNA expression levels of 15 cytosolic pattern recognition molecules in 11 solid organs of human and mice. Human organs revealed lower mRNA levels of most molecules as in spleen but at least 2-fold higher were inflammasome-related NOD, leucine-rich repeat and pyrin domain-containing protein 1-3 (NLRP1-3) and -12 in brain, LGP2, retinoic acid-inducible gene I (RIG-I) and NLRP10 in liver, NLRP10 in small intestine, LGP2, RIG-I, NAIP, NLRP2 and -3 in testis and RIG-I, NLRP2 and -10 in muscle. In mice, most organs also expressed lower mRNA levels compared with spleen. Only NLRP6 in liver, NAIP and NLRP6 in small intestine, LGP2, nucleotide-binding oligomerization domain 1 (NOD1), NLRP1, -2, -6, -10 and -12 in colon and MDA5, RIG-I, NLRC4, NOD1, -2, NLRP1, -2, -6, -10 and -12 mRNA levels in kidney were higher. Resting human and mouse monocytes and T cells expressed most molecules and produced IL-1 beta and CCL5/RANTES upon activation. However, murine monocytes strongly up-regulated, whereas human monocytes down-regulated receptor expression upon activation. These data suggest that the cell-type-, organ- and species-specific expression and regulation need to be considered in the design and interpretation of related studies.