CHARACTERIZATION OF A RED JUNGLE FOWL BY WHITE LEGHORN BACKCROSS REFERENCE POPULATION FOR MOLECULAR MAPPING OF THE CHICKEN GENOME
CHARACTERIZATION OF A RED JUNGLE FOWL BY WHITE LEGHORN BACKCROSS REFERENCE POPULATION FOR MOLECULAR MAPPING OF THE CHICKEN GENOME
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DOI：
10.3382/ps.0720334
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发表时间：
1993-02-01
期刊：
影响因子：
4.4
通讯作者：
DODGSON, JB
中科院分区：
文献类型：
--
作者：
CRITTENDEN, LB;PROVENCHER, L;DODGSON, JB
通过将部分近交的红原鸡 (JF) 品系与高度近交的白来航 (WL) 品系回交，产生了为鸡基因组分子遗传作图而设计的参考群体。选择亲本系以最大化它们之间的预期遗传多态性。将 JF 雄性与 WL 雌性杂交产生的两只全同胞 F1 雄性，分别与约 10 只 WL 雌性交配，产生 400 个后代。所有后代均根据控制颜色表型的三个基因座和六个血型基因座的分离进行分类，其中一些基因座已通过经典方法进行定位。除一个例外外，这 9 个基因座的分离与预期的 1:1 比例没有显着差异。至少储存了所有父母和后代的 20 mL 全血，为分子分析提供 DNA。通过Southern印迹与克隆基因以及随机扩增多态性DNA (RAPD)程序对亲本系和F1杂交的筛选揭示了大量亲本系特异性的分子标记。已完成对16个回交后代2个颜色位点、6个血型位点、16个克隆鸡基因检测位点、4个RAPD方法检测位点多态性的初步分析。 28 个位点中的 27 个位点的分离与预期的 1:1 比例没有显着差异，表明在每个位点检测到两个替代等位基因。检测到五对连锁基因座（P 小于或等于 0.01）。因此，该群体是多态性的，并且为两种类型的分子探针提供了简单的分离，为鸡基因组的协作管理提供了良好的资源。
A reference population designed for molecular genetic mapping of the chicken genome was produced by backcrossing a partially inbred Red Jungle Fowl (JF) line to a highly inbred White Leghorn (WL) line. The parental lines were chosen to maximize the expected genetic polymorphisms between them. Two full-sib F1 males, produced by crossing a JF male with a WL female, were each individually mated to about 10 WL females to produce 400 progeny. All the progeny were classified for segregation of three loci controlling color phenotype and six blood group loci, some of which have been mapped by classical methods. Segregation of these nine loci did not differ significantly from the expected 1:1 ratio with one exception. At least 20 mL of whole blood was stored from all the parents and progeny to provide DNA for molecular analysis. Screening of the parental lines and F1 crosses by Southern blot with cloned genes and by the random amplified polymorphic DNA (RAPD) procedure revealed a large number of molecular markers that were parental line-specific. A preliminary analysis of 16 backcross progeny classified for polymorphisms at 2 color loci, 6 blood group loci, 16 loci detected by cloned chicken genes, and 4 loci detected by the RAPD method has been completed. Segregation at 27 out of 28 loci did not differ significantly from the expected 1:1 ratio, showing that two alternative alleles were detected at each locus. Five pairs of linked loci were detected (P less-than-or-equal-to .01). Thus, this population is polymorphic and gives simple segregation for two types of molecular probes, providing a good resource for collaborative ma in of the chicken genome.