A mathematical model of lateral flow bioreactions applied to sandwich assays
A mathematical model of lateral flow bioreactions applied to sandwich assays
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DOI：
10.1016/j.ab.2003.07.011
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发表时间：
2003-11-01
影响因子：
2.9
通讯作者：
Bau, HH
中科院分区：
文献类型：
--
作者：
Qian, SZ;Bau, HH
横向流动(LF)生物检测器有助于在护理点低成本、快速识别各种分析物。LF细胞由一层多孔膜组成，膜上含有不同位置的固定化配体。通过毛细管力的作用，样品和报告粒子被输送到配体位置。然后扫描或探测LF膜，并测量报告粒子的浓度。建立了夹心分析的数学模型，并用它研究了低频装置在不同操作条件下的性能。该模型提供了对某些实验观察的洞察，包括在高目标分析物浓度下检测到的信号水平的降低。此外，该模型还可用于快速、廉价地测试各种工作条件，辅助器件设计，并优化低频器件的性能。(C)2003 Elsevier Inc.保留所有权利。
Lateral flow (LF) biodetectors facilitate low-cost, rapid identification of various analytes at the point of care. The LF cell consists of a porous membrane containing immobilized ligands at various locations. Through the action of capillary forces, samples and reporter particles are transported to the ligand sites. The LF membrane is then scanned or probed, and the concentration of reporter particles is measured. A mathematical model for sandwich assays is constructed and used to study the performance of the LF device under various operating conditions. The model provides insights into certain experimental observations including the reduction in the level of the detected signal at high target analyte concentrations. Furthermore, the model can be used to test rapidly and inexpensively various operating conditions, assist in the device's design, and optimize the performance of the LF device. (C) 2003 Elsevier Inc. All rights reserved.