Functional analysis of pheromone receptor repertoire in the fall armyworm, Spodoptera frugiperda
Functional analysis of pheromone receptor repertoire in the fall armyworm, Spodoptera frugiperda
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DOI：
10.1002/ps.6831
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发表时间：
2022-02-24
影响因子：
4.1
通讯作者：
Wang，Chen-Zhu
中科院分区：
文献类型：
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作者：
Guo，Hao;Gong，Xin-Lin;Wang，Chen-Zhu
草地秋粘虫Spodoptera frugiperda（J.E.史密斯），是一种多食性蛾类，正在地球仪各地蔓延。它使用（Z）-9-十四碳烯乙酸酯（Z9 - 14：Ac）和（Z）-7-十二碳烯乙酸酯（Z7 - 12：Ac）（100：3.9）作为基本的性信息素成分。然而，我们的理解的分子基础的信息素检测的S。在此，我们通过转录组测序鉴定了6个PR，即SfruOR 6、11、13、16、56和62。随后，我们在果蝇OR 67 d神经元中异源表达了它们，并用大量的性信息素和类似物确定了它们的反应谱。其中，表达SfruOR 13的神经元对主要性信息素成分Z9 - 14：Ac有强烈反应，但对（Z，E）-9，12-十四碳二烯乙酸酯（Z9，E12 - 14：Ac）也有强烈反应，对（Z）-9-十二碳烯乙酸酯（Z9 - 12：Ac）也有微弱反应。SfruOR 56和SfruOR 62都是针对次要性信息素成分Z7 - 12：Ac进行调节的，具有不同的强度和灵敏度。此外，SfruOR 6仅被Z9、E12 - 14：Ac激活，而SfruOR 16被（Z）-9-十四烯醇（Z9 - 14：OH）和（Z）-9-十四烯醛（Z9 - 14：Ald）激活。然而，表达SfruOR 11的OR 67 d神经元对所有测试的化合物保持沉默，这是在夜蛾科物种的OR 11进化枝中常见的现象。其次，利用单感器记录技术，我们对雄性触角上的四种感器类型进行了表征，即A、B、C和D型，它们与PR的配体相调谐，从而证实了S。frugiperdauses SfruOR 56和SfruOR 62检测Z7 - 12：Ac。最后，使用风洞试验，我们证明了Z9，E12 - 14：Ac和Z9 - 14：OH作为拮抗剂的性信息素。结论我们已经去乙酰化的5个PR和特征的4种类型的感受器负责检测信息素化合物，提供洞察到外周编码的性信息素在草地贪夜蛾。
BACKGROUNDThe fall armyworm,Spodoptera frugiperda(J. E. Smith), is a polyphagous moth species that is spreading all around the globe. It uses (Z)‐9‐tetradecenyl acetate (Z9‐14:Ac) and (Z)‐7‐dodecenyl acetate (Z7‐12:Ac) (100:3.9) as essential sex pheromone components. However, our understanding of the molecular basis of pheromone detection ofS. frugiperdais still incomplete.RESULTSHerein, we identified sixPRs, i.e.SfruOR6,11,13,16,56, and62, by transcriptome sequencing. Subsequently, we heterologously expressed them inDrosophilaOR67d neurons and determined their response spectra with a large panel of sex pheromones and analogs. Among them, SfruOR13‐expressing neurons strongly respond to the major sex pheromone component Z9‐14:Ac, but also comparably to (Z,E)‐9,12‐tetradecadienyl acetate (Z9,E12‐14:Ac) and weakly to (Z)‐9‐dodecenyl acetate (Z9‐12:Ac). Both SfruOR56 and SfruOR62 are specifically tuned to the minor sex pheromone component Z7‐12:Ac with varying intensities and sensitivities. In addition, SfruOR6 is activated only by Z9,E12‐14:Ac, and SfruOR16 by both (Z)‐9‐tetradecenol (Z9‐14:OH) and (Z)‐9‐tetradecenal (Z9‐14:Ald). However, the OR67d neurons expressing SfruOR11 remain silent to all compounds tested, a phenomenon commonly found in the OR11 clade of Noctuidae species. Next, using single sensillum recording, we characterized four sensilla types on the antennae of males, namely A, B, C and D types that are tuned to the ligands of PRs, thereby confirming thatS. frugiperdauses both SfruOR56 and SfruOR62 to detect Z7‐12:Ac. Finally, using wind tunnel assay, we demonstrate that both Z9,E12‐14:Ac and Z9‐14:OH act as antagonists to the sex pheromone.CONCLUSIONWe have deorphanized five PRs and characterized four types of sensilla responsible for the detection of pheromone compounds, providing insights into the peripheral encoding of sex pheromones inS. frugiperda.