Histological structure of duodenum in gilts receiving low doses of zearalenone and deoxynivalenol in feed
Histological structure of duodenum in gilts receiving low doses of zearalenone and deoxynivalenol in feed
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DOI：
10.1016/j.etp.2015.11.008
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发表时间：
2016-02-01
影响因子：
--
通讯作者：
Gajecki, Maciej
中科院分区：
文献类型：
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作者：
Lewczuk, Bogdan;Przybylska-Gornowicz, Barbara;Gajecki, Maciej
脱氧雪腐镰刀菌烯醇（DON）和玉米赤霉烯酮（ZEN）是由镰刀菌科的微真菌产生的最常见的真菌毒素。它们被认为是影响人类和动物健康以及牲畜生产力的重要因素。本研究的目的是确定低剂量的这些霉菌毒素对猪十二指肠组织结构的影响。对72头初始体重约为25 kg的后备母猪进行了研究，分为4个相等组。第I组口服ZEN（40 μ g/kg BW），第II-DON（12 μ g/kg BW），第III组ZEN（40 μ g/kg BW）和DON（12 μ g/kg BW），以及第IV组-载体。处理后1、2、3、4、5和6周，将猪处死，并制备十二指肠样品用于组织学研究。将载玻片数字化并进行形态计量学分析。DON和ZEN处理不改变粘膜的结构或上皮中杯状细胞和吸附细胞的比例。DON的施用诱导粘膜上皮中淋巴细胞数量的增加。两种真菌毒素单独或一起给药，增加了固有层中淋巴细胞、浆细胞和巨噬细胞的数量，并伴有黑褐色颗粒。DON和ZEN诱发的防御系统细胞数量变化的时程不同。总之，饮食暴露于低剂量的镰刀菌真菌毒素应被视为小肠亚临床炎症的一个重要危险因素。(C)2015爱思唯尔有限公司All rights reserved.
Deoxynivalenol (DON) and zearalenone (ZEN), produced by microfungi of the Fusarium family, are among the most commonly occurring mycotoxins. They are considered important factors affecting human and animal health as well as livestock productivity. The aim of this study was to determine the effect of low doses of these mycotoxins on the histological structure of the pig duodenum. The study was performed on 72 gilts, with initial weights of approximately 25 kg, divided into 4 equal groups. Group I received per os ZEN (40 mu g/kg BW), group II-DON (12 mu g/kg BW), group III ZEN (40 mu g/kg BW) and DON (12 mu gikg BW), and group IV-vehicle. The pigs were killed after 1, 2, 3, 4, 5 and 6 weeks of the treatment, and the duodenum samples were prepared for histological investigations. The slides were digitalized and subjected to morphometrical analysis. The treatment with DON and ZEN did not change the architecture of the mucosa or the ratio between goblet and adsorptive cells in the epithelium. The administration of DON induced an increase in the number of lymphocytes in the mucosal epithelium. Both mycotoxins, administered alone or together, increased the quantity of lymphocytes, plasma cells and macrophages with black-brown granules in the lamina propria. The time-courses of changes in the number of defense system cells evoked by DON and ZEN were different. In conclusion, dietary exposure to low doses of Fusarium mycotoxins should be considered an important risk factor for subclinical inflammation in the small intestine. (C) 2015 Elsevier GmbH. All rights reserved.