Effects of Low-Level Laser Irradiation on Rat Skeletal Muscle Injury after Eccentric Exercise
Effects of Low-Level Laser Irradiation on Rat Skeletal Muscle Injury after Eccentric Exercise
复制标题
低强度激光照射对离心运动后大鼠骨骼肌损伤的影响
DOI：
10.1089/pho.2008.2443
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发表时间：
2009-12-01
影响因子：
--
通讯作者：
Yuan, Jian-Qin
中科院分区：
文献类型：
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作者：
Liu, Xiao-Guang;Zhou, Yong-Jian;Yuan, Jian-Qin
背景与目的：光生物调节对迟发性肌肉酸痛的影响尚不清楚。这项研究是首次使用动物模型对这种治疗进行调查。方法：将72只Sprague-Dawley大鼠随机分为5组：久坐对照组、运动对照组和运动+激光组。采用下坡运动诱导腓肠肌损伤。运动+激光组损伤肌肉分别在运动后立即和18、42 h以12、28、43 J/cm(2)的速度照射He-Ne激光。运动后24和48 h分别进行组织学检查和血清肌酸激酶（CK）、肌肉超氧化物歧化酶（SOD）和丙二醛（MDA）分析。结果：运动对照组大鼠下坡跑后腓肠肌出现明显炎症反应，血清CK活性和肌肉MDA水平明显升高。43 J/cm(2)的He-Ne激光在运动后24和48 h均能抑制肌肉炎症，显著提高肌肉SOD活性，显著降低血清CK活性和肌肉MDA水平，而12和28 J/cm(2)的He-Ne激光仅在运动后48 h轻度抑制肌肉炎症，显著降低血清CK活性（P < 0.05）。结论：低水平氦氖激光治疗可通过增强肌肉抗氧化能力和减少炎症反应来治疗偏心运动性大鼠肌肉损伤。光生物调节是剂量依赖性的，43 J/cm(2)的剂量是最有效的。
Background and Objective: The effect of photobiomodulation on delayed onset muscle soreness remains unknown. This study represents the first investigation of this treatment using an animal model. Methods: Seventy-two Sprague-Dawley rats were randomly divided into five groups: sedentary control group, exercise control group and three exercise-plus-laser groups. Downhill running was used to induce muscle injury in the gastrocnemius muscle. He-Ne laser irradiations were administered to the injured muscles immediately and at 18 and 42 h after exercise in the three exercise-plus-laser groups at 12, 28, and 43 J/cm(2), respectively. Histological examination and serum creatine kinase (CK), muscle superoxide dismutase (SOD) and malondialdehyde (MDA) analyses were done at 24 and 48 h after exercise. Results: The exercise control group exhibited a marked inflammation in the gastrocnemius muscle and significant elevations in serum CK activity and muscle MDA level after downhill running. He-Ne laser irradiation at 43 J/cm(2) inhibited muscle inflammation, significantly enhanced muscle SOD activity and significantly reduced serum CK activity and muscle MDA level at both 24 and 48 h after exercise, whereas the irradiation at 12 or 28 J/cm(2) slightly inhibited muscle inflammation and significantly reduced serum CK activity at 48 h after exercise only (P < 0.05). Conclusions: Low-level He-Ne laser therapy could exert therapeutic effects on eccentric exercise-induced rat muscle injury through enhancing muscle anti-oxidative capacity and reducing the inflammatory reaction. The photobiomodulation was dose-dependent, and the 43 J/cm(2) dose was the most efficient among the doses used.