Unique and selective mitogenic effects of vanadate on SV40-transformed cells.
Unique and selective mitogenic effects of vanadate on SV40-transformed cells.
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钒酸盐对 SV40 转化细胞具有独特且选择性的促有丝分裂作用。
DOI：
10.1007/bf01075919
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发表时间：
1995
影响因子：
4.3
通讯作者：
Scott,RE
中科院分区：
文献类型：
--
作者：
Wang,H;Scott,RE
钒酸盐和胰岛素均作为SV 40转化的3 T3 T细胞（命名为CSV 3 -1）的独特完全有丝分裂原发挥作用，但不作为非转化的3 T3 T细胞的有丝分裂原发挥作用。钒酸盐和胰岛素对CSV 3 -1细胞的促有丝分裂作用是通过不同的信号转导机制介导的。例如，钒酸盐既不刺激胰岛素受体β亚基的酪氨酸磷酸化，也不刺激170 kDa胰岛素受体底物-1。相反，钒酸盐诱导55和64 kDa蛋白的酪氨酸磷酸化的显着增加，这在胰岛素刺激的CSV 3 -1细胞中未观察到。也许最有趣的是，钒酸盐诱导的有丝分裂与选择性诱导c-jun和junB表达相关，而不显著诱导c-fosorc-myc。此外，用染料木素处理CSV 3 -1细胞可消除钒酸盐对蛋白酪氨酸磷酸化和c-jun诱导的作用。这些和相关的数据表明，蛋白酪氨酸磷酸化和c-jun和junB表达的调制可能在介导钒酸盐诱导的SV 40转化细胞有丝分裂中发挥关键作用。
Vanadate and insulin both function as unique complete mitogens for SV40-transformed 3T3T cells, designated CSV3-1, but not for nontransformed 3T3T cells. The mitogenic effects induced by vanadate and insulin in CSV3-1 cells are mediated by different signaling mechanisms. For example, vanadate does not stimulate the tyrosine phosphorylation of the insulin receptor β-subunit nor the 170 kDa insulin receptor substrate-1. Instead, vanadate induces a marked increase in tyrosine phosphorylation of 55 and 64 kDa proteins that is not observed in insulin-stimulated CSV3-1 cells. Perhaps most interestingly, vanadate-induced mitogenesis is associated with the selective induction ofc-junandjunBexpression without significantly inducingc-fosorc-myc. Furthermore, treatment of CSV3-1 cells with genistein abolishes the effects of vanadate on protein tyrosine phosphorylation andc-juninduction. These and related data suggest that modulation of protein tyrosine phosphorylation andc-junandjunBexpression may serve the critical roles in mediating vanadate-induced mitogenesis in SV40-transformed cells.