Histological and transcriptional effects of androstenedione in adult zebrafish ( Danio rerio )
Histological and transcriptional effects of androstenedione in adult zebrafish ( Danio rerio )
复制标题
雄烯二酮对成年斑马鱼（Danio rerio）的组织学和转录作用
DOI：
10.1002/tox.23064
复制
发表时间：
2021
影响因子：
4.5
通讯作者：
Meina Duan
中科院分区：
文献类型：
--
作者：
Yan-Qiu Liang;Chang-Gui Pan;Linchun Xie;Jialiang Huo;Jiahao Yang;Tianhong Lan;Zhongdian Dong;Meina Duan
雄烯二酮（AED）作为一种天然雄激素，广泛存在于水生环境中，可能对水生生物造成潜在的危害。本文研究了AED对斑马鱼（Danio rerio）性腺组织学的影响，以及与下丘脑-垂体-性腺（HPG）轴、生殖细胞分化和性别分化相关的34个基因的mRNA表达水平。成年斑马鱼暴露于溶剂对照和三种测量浓度（0.2、2.3和23.7 μg/L AED）60天。结果表明，AED对卵巢和睾丸无明显的组织学效应。在所研究的基因中，2.3或23.7 μg/L的AED显著增加了雌性卵巢中amh和cyp 11 c1基因的转录表达水平。不同浓度的AED均显著抑制雄性大鼠脑内gnrh 3、atf 4 b1和cyp 19 a1 b的mRNA表达。在雄性大鼠睾丸中，2.3 μg/L的AED可诱导sox 9 b基因的表达，而23.7 μg/L的AED可下调nr 5a 1b基因的表达。这些观察到的转录变化表明，AED可以在斑马鱼中产生潜在的雄激素效应。
As a natural androgen, androstenedione (AED) may pose potential risks to aquatic organisms due to its ubiquitousness in aquatic environments. Here we assessed the adverse effects of AED on histology of gonads, as well as mRNA expression levels of 34 genes concerned with hypothalamic-pituitary-gonadal (HPG) axis, germ-cell differentiation and sex differentiation in zebrafish (Danio rerio). Adult zebrafish were exposed to solvent control and three measured concentrations of 0.2, 2.3 and 23.7 μg/L AED for 60 days. The results showed that AED did not induce any obvious histological effects in the ovaries and testes. Of the investigated genes, transcriptional expression levels of amh and cyp11c1 genes in the ovaries of females were significantly increased by AED at 2.3 or 23.7 μg/L. However, different exposure concentrations of AED significantly inhibited mRNA expression of gnrh3, atf4b1 and cyp19a1b in the brain of males. In the testes of males, AED at 2.3 μg/L led to a significant induction of sox9b gene, but it at 23.7 μg/L down-regulated nr5a1b gene. These observed transcriptional changes indicated that AED could pose potential androgenic effects in zebrafish.