Cluster-analysis & patterns of dissemination of multidrug resistance among clinical strains of Vibrio cholerae in Calcutta, India.
Cluster-analysis & patterns of dissemination of multidrug resistance among clinical strains of Vibrio cholerae in Calcutta, India.
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2000-09
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通讯作者：
T. Ramamurthy;K. Rajendran;P. Garg;T. Shimada;A. Basu;N. Chowdhury;R. Nandy;S. Yamasaki;S. Bhattacharya;Y. Takeda;G. Nair
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作者：
T. Ramamurthy;K. Rajendran;P. Garg;T. Shimada;A. Basu;N. Chowdhury;R. Nandy;S. Yamasaki;S. Bhattacharya;Y. Takeda;G. Nair
背景与方法在加尔各答，霍乱弧菌的耐药性已经被监测了好几年。为了解不同血清型霍乱弧菌多药耐药（MDR）的变化趋势，并进行软件辅助聚类分析，本研究进行了。方法分析从加尔各答传染病医院收治的霍乱和“霍乱样”腹泻患者中分离的菌株。从1992年至1997年分离的840株霍乱弧菌对11种抗生素进行了敏感性试验。用SPSS软件进行聚类分析。结果耐药菌株多为耐药菌株，耐药谱逐年变化，耐药率呈波动趋势。采用聚类组合法对霍乱弧菌O 1、O 139和非O 1、非O 139血清群共119株不同的耐药谱进行了分析。在1993年和1994年期间，分别有53%的霍乱弧菌O 139和82%的霍乱弧菌O 1血清群表现出最多的新耐药模式。在1995 - 1997年期间，霍乱弧菌非O 1、非O 139的新耐药模式的频率一直很高（33-47%）。解释与结论除少数例外，耐药谱的优势一般不限于任何血清群。聚类分析描述了在随后几年中属于不同血清群的非O 1、非O 139霍乱弧菌中常见的一些耐药模式向O 139血清群的传播。在这项研究中，我们已经表明，霍乱弧菌菌株对几种抗生素具有耐药性，MDR谱不断变化。必须确定菌株的敏感性模式，以确定治疗霍乱的有效药物。
BACKGROUND & OBJECTIVES Antimicrobial resistance among Vibrio cholerae has been monitored for several years in Calcutta. To investigate the changing trends in multidrug resistance (MDR) among different serogroups of V. cholerae and to perform software assisted cluster analysis the current study was undertaken. METHODS Strains isolated from patients with cholera and "cholera-like" diarrhoea admitted in the Infectious Diseases Hospital, Calcutta were analysed. Eight hundred and forty V. cholerae strains isolated from 1992 through 1997 were tested for susceptibility to 11 antibiotics. Cluster analysis was done using SPSS software. RESULTS Most of the strains exhibited MDR with fluctuating trends as the resistance profile diverged each year. A total of 119 different resistance profiles exhibited by V. cholerae O1, O139 and non-O1, non-O139 serogroups were analysed by cluster combination method. During 1993 and 1994, 53 per cent of V. cholerae O139 and 82 per cent of V. cholerae O1 serogroups, respectively, exhibited maximal number of new resistance patterns. The frequency of new resistance patterns among V. cholerae non-O1, non-O139 was constantly high (33-47%) during 1995 to 1997. INTERPRETATION & CONCLUSIONS With a few exceptions, preponderance of the resistance profiles was generally not confined to any serogroup. The cluster analysis depicted dissemination of some of the resistance patterns commonly found among V. cholerae non-O1, non-O139 belonging to different serogroups to the O139 serogroup in the succeeding years. In this study we have shown that the V. cholerae strains are resistant to several antibiotics with constant change in the MDR profiles. It is imperative to define the susceptibility pattern of the strains to determine the effective drug of choice for the treatment of cholera.