Controlled coupling of peptides at their C-termini
Controlled coupling of peptides at their C-termini
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DOI：
10.1016/j.peptides.2008.12.008
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发表时间：
2009-04-01
期刊：
影响因子：
3
通讯作者：
Bak, Sonja
中科院分区：
文献类型：
--
作者：
Peschke, Bernd;Bak, Sonja
两种蛋白质的融合已成为生物技术中的重要工具。虽然生物技术方法可以很容易地产生C-末端到N-末端的融合化合物，但将两种蛋白质偶联到它们的每个C-末端的方法并不容易获得。在本文中，肽被用作较大蛋白质的模型。描述了一种方法，该方法利用由羧肽酶Y（CPY）催化的反应将不同的反应性手柄连接到它们的C-末端的可能性。可以将可以彼此反应的反应柄对连接到待偶联的每个肽。在第二步中，两个修饰的肽可以通过化学反应连接在一起，例如肟形成反应或叠氮化物与炔的铜（I）催化的[2+3]-环加成反应。(c)2008年爱思唯尔公司All rights reserved.
Fusion of two proteins has become an important tool in biotechnology. Whereas biotechnological methods easily can produce C-terminal to N-terminal fused compounds, methods to couple two proteins to each of their C-termini are not easily accessible. Herein, peptides are used as models for larger proteins. A method is described exploiting the possibility to attach different reactive handles to their C-termini using a reaction catalyzed by the enzyme carboxypeptidase Y (CPY). It is possible to attach pairs of reaction handles which can react with each other to each of the peptides to be coupled. In a second step, the two modified peptides can be linked together by a chemical reaction, such as an oxime-forming reaction or a copper(I) catalyzed [2+3]-cycloaddition reaction of an azide with an alkyne. (c) 2008 Elsevier Inc. All rights reserved.