Epigenomics: sequencing the methylome.
Epigenomics: sequencing the methylome.
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DOI：
10.1007/978-1-62703-281-0_3
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发表时间：
2013
影响因子：
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通讯作者：
M. Hirst
中科院分区：
文献类型：
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作者：
M. Hirst
DNA甲基化模式越来越多地通过利用大规模并行测序的方法进行调查。为检测DNA甲基化而开发的基于序列的分析方法大致可分为亲和富集法、化学转化法或酶限制法。从这些方法得到的DNA片段被统一地进行文库构建和大规模并行测序。序列读数随后与参考基因组进行比对，并接受专门的分析工具以提取潜在的甲基化签名。本章将概述这些新兴技术。
DNA methylation patterns are increasingly surveyed through methods that utilize massively parallel sequencing. Sequence-based assays developed to detect DNA methylation can be broadly divided into those that depend on affinity enrichment, chemical conversion, or enzymatic restriction. The DNA fragments resulting from these methods are uniformly subjected to library construction and massively parallel sequencing. The sequence reads are subsequently aligned to a reference genome and subjected to specialized analytical tools to extract the underlying methylation signature. This chapter will outline these emerging techniques.