Simple preparation method of PCR fragments for automated DNA sequencing
Simple preparation method of PCR fragments for automated DNA sequencing
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自动化DNA测序PCR片段的简单制备方法
DOI：
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发表时间：
1999
影响因子：
4
通讯作者：
J. Vuust
中科院分区：
文献类型：
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作者：
E. Høgdall;K. Boye;J. Vuust
为了找到一种简单而廉价的纯化方法，聚合酶链反应（PCR）反应前的循环测序反应，我们比较了商业系统与沉淀协议在我们的实验室进行。我们发现，特别是对于小的PCR产物，我们的方法比所比较的方法更成功。我们的沉淀方法也可以在循环测序反应之前用于较大的PCR片段。此外，它具有与众所周知的稀释法一样简单的优点;与稀释法相比，沉淀法去除了过量的引物以及可能的引物二聚体。J.细胞。73：433-436，1999.© 1999 Wiley利斯公司
In an effort to find a simple and inexpensive purification method of polymerase chain reaction (PCR) reaction before cycle sequencing reaction, we compared a commercial system with a precipitation protocol performed in our laboratory. We found that, particularly with small PCR products, our method works with greater success than the method compared. Our precipitation method may be used on a larger PCR fragment before cycle sequencing reaction as well. Furthermore, it has the advantage of being simple as the well‐known dilution method; in contrast to the dilution method, the precipitation method removes excess primers as well as possible primer dimers. J. Cell. Biochem. 73:433–436, 1999. © 1999 Wiley‐Liss, Inc.