Melinjo (Gnetum gnemon) seed extract consumption during lactation improved vasodilation and attenuated the development of hypertension in female offspring of fructose-fed pregnant rats.
Melinjo (Gnetum gnemon) seed extract consumption during lactation improved vasodilation and attenuated the development of hypertension in female offspring of fructose-fed pregnant rats.
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哺乳期间食用买麻藤种子提取物可以改善果糖喂养的怀孕大鼠雌性后代的血管舒张并减轻高血压的发展。
DOI：
10.1002/bdr2.1109
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Kurasaki M
中科院分区：
文献类型：
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作者：
Uson-Lopez RA;Kataoka S;Mukai Y;Sato S;Kurasaki M
背景果糖摄入量与包括高血压在内的代谢性疾病患病率增加相关。在怀孕的大鼠中，据报道果糖摄入对其后代的健康有不利影响。这项研究调查了妊娠期母体果糖消费的影响，如果补充melinjo种子提取物的母亲在哺乳期的饮食可以受益于后代在以后的life.MethodsPregnant大鼠被随机分为三组：未处理（CC），果糖治疗（FC），果糖和melinjo治疗（FM）。FC和FM组在妊娠期间通过饮用水的方式接受100 g/L的D（-）-果糖溶液，而CC组接受正常饮用水。在哺乳期，CC和FC组给予标准的商业实验室饮食，而FM组给予商业实验室饮食与0.1%的梅林果种子提取物。断奶后，给予后代正常饮用水和标准商业饮食，直到第17周。监测子代的血压，直至第16周。在第17周，后代被杀死，肾脏收集和analysed.ResultsThe水平磷酸化AMP活化蛋白激酶（pAMPK）在FM的17周的女性后代是显着高于FC和CC组相比。母体果糖摄入量下调了FC中一氧化氮合成酶的肾内皮亚型表达，而母体melinjo种子提取物摄入量维持了17周雌性后代FM中一氧化氮合成酶的肾内皮亚型表达。此外，母亲melinjo种子提取物的摄入量在哺乳期降低了收缩压FM的17周的雌性后代。ConclusionFemale后代更容易受到胎盘果糖和melinjo种子提取物的影响，这表明性别特异性敏感性。总之，我们的数据表明，在哺乳期间食用梅林果种子提取物改善了果糖喂养的妊娠大鼠的17周雌性后代的血管舒张并减轻了高血压的发展。出生缺陷研究110：27-34，2018。© 2017 Wiley Periodicals，Inc.
BackgroundFructose intake has been correlated with increased prevalence of metabolic disorders including hypertension. In pregnant rats, fructose intake has been reported to have adverse effects on the health of its offspring. This study investigated the effects of gestational maternal fructose consumption and if supplementation with melinjo seed extracts to the maternal diet during lactation could benefit the offspring in later life.MethodsPregnant rats were randomly divided into three groups: untreated (CC), fructose‐treated (FC), and fructose and melinjo‐treated (FM). FC and FM groups received 100 g/L of D(‐)‐fructose solution by means of the drinking water during gestation while CC received normal drinking water. During lactation, CC and FC groups were given standard commercial laboratory diet, while the FM group was given commercial laboratory diet with 0.1% melinjo seed extracts. After weaning, the offspring were given normal drinking water and standard commercial diet until week 17. The blood pressure of the offspring was monitored until the 16th week. During week 17, the offspring were killed, and the kidneys were collected and analyzed.ResultsThe level of renal phosphorylated AMP‐activated protein kinase (pAMPK) in FM of 17‐week female offspring was significantly higher compared with FC and CC groups. Maternal fructose intake down‐regulated the renal endothelial isoform of nitric oxide synthetase expression in FC and maternal melinjo seed extract consumption maintained renal endothelial isoform of nitric oxide synthetase expression in FM of 17‐week female offspring. In addition, maternal melinjo seed extract intake during lactation lowered the systolic blood pressure in FM of 17‐week female offspring.ConclusionFemale offspring were more vulnerable to the effects of placental fructose and melinjo seed extracts, suggesting sex‐specific sensitivities. In summary, our data show that melinjo seed extract consumption during lactation improved vasodilation and attenuated the development of hypertension in the 17‐week female offspring of fructose‐fed pregnant rats. Birth Defects Research 110:27–34, 2018. © 2017 Wiley Periodicals, Inc.