QUANTITATIVE MEASUREMENT OF THE ERYTHROCYTIC AND GRANULOCYTIC CELLS OF THE MARROW AND BLOOD
QUANTITATIVE MEASUREMENT OF THE ERYTHROCYTIC AND GRANULOCYTIC CELLS OF THE MARROW AND BLOOD
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DOI：
10.1172/jci103750
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发表时间：
1958-01-01
影响因子：
15.9
通讯作者：
FINCH, CA
中科院分区：
文献类型：
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作者：
DONOHUE, DM;REIFF, RH;FINCH, CA
材料和方法在体重在200至300克之间的Sprague-Dawley大鼠上进行研究，新西兰白色家兔，体重2 - 33只/Kg，和2至3 Kg。雄性恒河猴所有动物均给予约15 mg。每公斤至少在研究前两周以铁右旋糖酐2形式补充铁，以确保有足够的铁储备。仅限于血红蛋白水平高于13 Gm的动物。用了兔子的网织红细胞计数必须低于4%，猴子和大鼠的网织红细胞计数必须低于3%，才被认为适合研究。手术前，这些患者白细胞计数正常，无发热，但血浆铁值通常低于正常。手术当天早晨，红细胞计数高于4.5 × 106，网织红细胞计数低于2%，在过去的六个月里没有输血。
MATERIALS AND METHODSStudies were performed on Sprague-Dawley rats weighing between 200 to 300 Gm., New Zealand white rabbits weighing 2 to 33/Kg., and 2 to 3 Kg. male Rhesus monkeys. All animals were given approximately 15 mg. per Kg. of iron as iron dextran2 at least two weeks before the study to insure adequate iron stores. Only those animals with hemoglobin levels above 13 Gm. were used. Reticulocyte counts in rabbits were required to be below 4 per cent and those of monkeys and rats below 3 per cent before the animals were considered suitable for study.Clinical studies were performed on male patients with controlled tuberculosis who were being subjected to thoracotomy. Prior to operation, these patients had normal leukocyte counts and were afebrile, but plasma iron values were usually subnormal. On the morning of operation their red counts were above 4.5 X 106; their reticulocyte counts were less than 2 per cent. They had not been transfused during the previous six months.