Cloning and characterization of mammalian homologs of the Drosophila dunce+ gene.
Cloning and characterization of mammalian homologs of the Drosophila dunce+ gene.
复制标题
果蝇 dunce 基因的哺乳动物同源物的克隆和表征。
DOI：
10.1073/pnas.86.10.3604
复制
发表时间：
1989
影响因子：
11.1
通讯作者：
Myres,J
中科院分区：
文献类型：
--
作者：
Davis,RL;Takayasu,H;Eberwine,M;Myres,J
代表Drosophila dunce+（dnc+）基因（cAMP磷酸二酯酶（PDEase）的结构基因）的探针可检测许多不同生物（包括小鼠、大鼠和人类）中的同源序列。基因组和cDNA克隆的果蝇dnc+基因的同源物进行了分离，从大鼠的图书馆和特点。该基因被命名为ratdnc-1。一个cDNA克隆定义了约1.8个PDE（kb）的大开放阅读框，预测了610个氨基酸的蛋白质序列，其与在大多数其它PDE酶中发现的约275个残基的保守结构域具有显著同源性。在该结构域内与果蝇cAMP PDE酶的氨基酸同一性值为惊人的75%。其他的cDNA克隆显示出与该cDNA克隆接近预测的N末端的序列差异块，表明可能存在由来自ratdnc-1的选择性剪接的信使RNA编码的相关酶家族。基因组印迹实验表明至少存在一个与ratdnc-1同源的其他大鼠基因。与ratdnc-1同源的RNA在组织之间的大小是异质的，心脏含有4.4 kb的主要转录物，而大脑含有4.0 kb的主要转录物。潜在的身份的ratdnc-1基因与已知的PDEases的产品进行了讨论。
A probe representing the Drosophila dunce+ (dnc+) gene, the structural gene for a cAMP phosphodiesterase (PDEase), detects homologous sequences in many different organisms, including mouse, rat, and human. Genomic and cDNA clones representing a homolog of the Drosophila dnc+ gene were isolated from rat libraries and characterized. This gene has been named ratdnc-1. One cDNA clone defines a large open reading frame of approximately 1.8 kilobases (kb), predicting a protein sequence of 610 amino acids with significant homology to a conserved domain of approximately 275 residues found in most other PDEases. The amino acid identity value to the Drosophila cAMP PDEase within this domain is a striking 75%. Other cDNA clones show blocks of sequence divergence from this cDNA clone close to the predicted N terminus, indicating the potential existence of a family of related enzymes encoded by alternatively spliced messenger RNAs from ratdnc-1. Genomic blotting experiments suggest the existence of at least one other rat gene with homology to ratdnc-1. RNAs homologous to ratdnc-1 are heterogeneous in size between tissues, with heart containing a major transcript of 4.4 kb and brain one of 4.0 kb. The potential identity of the product of the ratdnc-1 gene with known PDEases is discussed.