Differential expression of proteins involved in energy production along the crypt-villus axis in early-weaning pig small intestine
Differential expression of proteins involved in energy production along the crypt-villus axis in early-weaning pig small intestine
复制标题
早期断奶猪小肠隐窝-绒毛轴能量产生相关蛋白的差异表达
DOI：
10.1152/ajpgi.00095.2015
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发表时间：
2015-08-15
影响因子：
4.5
通讯作者：
Yin, Yulong
中科院分区：
文献类型：
--
作者：
Xiong, Xia;Yang, Huansheng;Yin, Yulong
仔猪断奶反映了肠道功能障碍和萎缩，影响了肠上皮细胞的生理状态。然而，很少有研究定义早期断奶仔猪沿隐窝-绒毛轴肠细胞的生理状态。实验共使用8窝16头仔猪。一组仔猪由母猪哺乳直至 21 天，另一组在 14 天时断奶，然后饲喂教槽饲料而不是母乳，持续 7 天。 21天时处死仔猪，解剖空肠段。沿隐窝-绒毛轴顺序分离空肠上皮细胞后，通过同量异位标签进行相对和绝对定量分析，并通过Western blotting测定断奶组或哺乳组空肠上皮细胞中雷帕霉素信号通路哺乳动物靶标蛋白和增殖细胞核抗原丰度。鉴定并分析了三个细胞组分中的差异蛋白。结果显示，早期断奶组的上、中绒毛中参与三羧酸循环、β-氧化和糖酵解途径的蛋白质显着下调。然而，参与糖酵解的蛋白质在隐窝细胞中显着上调。此外，Western blot分析显示，早期断奶组哺乳动物雷帕霉素通路相关蛋白表达量降低（P < 0.05）。目前的结果表明，早期断奶对沿空肠隐窝绒毛轴参与肠细胞能量产生的蛋白质的表达产生差异性影响。
Weaning of piglets reflects intestinal dysfunction and atrophy and affected the physiological state of enterocytes. However, few studies have defined physiological state of enterocytes along the crypt-villus axis in early-weaning piglets. A total of 16 piglets from 8 litters were used in the experiment. One group of piglets was nursed by sows until age 21 days, and another group was weaned at age 14 days and then fed creep feed instead of breast milk for 7 days. Piglets were killed at 21 days, and the jejunum segments were dissected. After sequential isolation of jejunum epithelial cells along the crypt-villus axis, their proteins were analyzed through the isobaric tags for relative and absolute quantification, and proteins involved in the mammalian target of rapamycin signaling pathway and proliferating cell nuclear antigen abundances in jejunal epithelial cells of weaning or suckling group were determined by Western blotting. The differential proteins in three cell fractions were identified and analyzed. The results showed that proteins involved in the tricarboxylic acid cycle, beta-oxidation, and the glycolysis pathway were significantly downregulated in the upper and middle villus of the early-weaned group. However, proteins involved in glycolysis were significantly upregulated in crypt cells. In addition, Western blot analysis showed that the expression of mammalian target of rapamycin pathway-related proteins was decreased (P < 0.05) in the early-weaned group. The present results showed that early-weaning differentially affect the expression of proteins involved in energy production of enterocytes along the jejunal crypt-villus axis.