Shape anisotropy induces rotations in optically trapped red blood cells
Shape anisotropy induces rotations in optically trapped red blood cells
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DOI：
10.1117/1.3430732
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发表时间：
2010-07-01
影响因子：
3.5
通讯作者：
Mathur, Deepak
中科院分区：
文献类型：
--
作者：
Bambardekar, Kapil;Dharmadhikari, Jayashree A.;Mathur, Deepak
用实验和理论相结合的方法研究了单光束光阱中红细胞的转动行为。我们通过改变活细胞漂浮的悬浮介质的性质来诱导红细胞的形状变化。我们发现，某些形状各向异性导致光学囚禁晶胞的旋转。事实上，即使是正常的(健康的)红细胞，也可以通过改变细胞所受的渗透压力，使用线偏振捕获光来旋转。研究发现，高渗胁迫可诱导细胞形态各向异性。我们还探讨了介质的粘度对细胞旋转的影响。观测到的旋转是使用朗之万型运动方程模拟的，该方程考虑了红细胞在介质中旋转时产生的摩擦力。我们观察到我们的测量数据和计算结果之间有很好的相关性。(C)2010年光学仪器工程师学会。[DOI：10.1117/1.3430732]
A combined experimental and theoretical study is carried out to probe the rotational behavior of red blood cells (RBCs) in a single beam optical trap. We induce shape changes in RBCs by altering the properties of the suspension medium in which live cells float. We find that certain shape anisotropies result in the rotation of optically trapped cells. Indeed, even normal (healthy) RBCs can be made to rotate using linearly polarized trapping light by altering the osmotic stress the cells are subjected to. Hyperosmotic stress is found to induce shape anisotropies. We also probe the effect of the medium's viscosity on cell rotation. The observed rotations are modeled using a Langevin-type equation of motion that takes into account frictional forces that are generated as RBCs rotate in the medium. We observe good correlation between our measured data and calculated results. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3430732]