Expression patterns of microRNAs 155 and 451 during normal human erythropoiesis
Expression patterns of microRNAs 155 and 451 during normal human erythropoiesis
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DOI：
10.1016/j.bbrc.2007.10.077
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发表时间：
2007-12-21
影响因子：
3.1
通讯作者：
Umemura, Tsukuru
中科院分区：
文献类型：
--
作者：
Masaki, Shizuka;Ohtsuka, Rie;Umemura, Tsukuru
为了阐明microRNA（miRNAs）在红细胞生成中的作用，在液体培养系统中分析了纯化的正常人红系祖细胞分化过程中miR-155、miR-221、miR-223和miR-451的表达。细胞数量增加近500倍，并分化为联苯胺阳性的成熟成红细胞。使用定量实时聚合酶链反应分析miRNA表达显示，在12天的培养期间，miR-155的表达水平降低约200倍，而iniR-451的表达增加约270倍。观察到miR-221和miR-223的中度下调。从正常人外周血中获得的MiR-451在红细胞中的表达比在粒细胞中高约10（4）倍。这些观察结果表明，miR-155和miR-451是正常红细胞分化的关键分子，这两种miRNA的定量测定可能是分析病理性红细胞生成的相关方法。(C)2007年爱思唯尔公司All rights reserved.
To clarify the roles of microRNAs (miRNAs) in erythropoiesis, the expression of miR-155, miR-221, miR-223, and miR-451 were analyzed during the differentiation of purified normal human erythroid progenitors in a liquid culture system. Cells increased almost 500-fold in a number, and differentiated to benzidine-positive mature erythroblasts. Analyses of miRNA expression using the quantitative real-time polymerase chain reaction showed that the expression level of miR-155 decreased about 200-fold, and that the expression of iniR-451 increased about 270-fold during 12 days of cultures. A moderate down-regulation of miR-221 and miR-223 was observed. MiR-451 was expressed in red blood cells about 10(4)-fold more than in granulocytes, obtained from normal human peripheral blood. These observations suggest that miR-155 and miR-451 are key molecules for normal erythroid differentiation, and that quantitative assays of the two miRNAs may be a relevant method for analyzing pathological erythropoiesis. (C) 2007 Elsevier Inc. All rights reserved.