BIOSYNTHESIS OF URIDINE DIPHOSPHATE D-XYLOSE .2. URIDINE DIPHOSPHATE D-GLUCURONATE CARBOXY-LYASE OF CRYPTOCOCCUS LAURENTII
BIOSYNTHESIS OF URIDINE DIPHOSPHATE D-XYLOSE .2. URIDINE DIPHOSPHATE D-GLUCURONATE CARBOXY-LYASE OF CRYPTOCOCCUS LAURENTII
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DOI：
10.1021/bi00865a024
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发表时间：
1966-01-01
期刊：
影响因子：
2.9
通讯作者：
FEINGOLD, DS
中科院分区：
文献类型：
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作者：
ANKEL, H;FEINGOLD, DS
劳伦隐球菌的尿苷二磷酸 D-葡萄糖醛酸羧基裂解酶已被部分纯化，并对其性质进行了研究。尿苷二磷酸、D-木糖和二氧化碳是酶作用的唯一产物。最适pH值在7.0至7.5之间。尿苷二磷酸 D-葡萄糖醛酸的 Km 为 1.1 x 10-3 [图片]；该酶对烟酰胺腺嘌呤二核苷酸（NAD）有绝对且特定的需求； Ka = 3 x 10-6 [图片]。还原型烟酰胺腺嘌呤二核苷酸与 NAD 竞争性抑制； Ki = 2 x 10-6 [图片]。该酶被对汞苯甲酸酯抑制；半胱氨酸可逆转这种抑制作用。当尿苷二磷酸 D-葡萄糖醛酸-3-t 或-4-t 被酶脱羧时，标记被保留。
Uridine diphosphate D-glucuronate carboxy-lyase of Cryptococcus laurentii has been partially purified and its properties have been investigated. Uridine diphosphate D-xylose and carbon dioxide are the only products of enzyme action. The pH optimum is between 7.0 and 7.5. Km for uridine diphosphate D-glucuronic acid is 1.1 x 10-3 [image]; the enzyme has an absolute and specific requirement for nicotinamide-adenine dinucleotide (NAD); Ka = 3 x 10-6 [image]. Reduced nicotinamide-adenine dinucleotide inhibits competitively with NAD; Ki = 2 x 10-6 [image]. The enzyme is inhibited by p-mercuribenzoate; the inhibition is reversed by cysteine. Label is retained when uridine diphosphate D-glucuronate-3-t or -4-t is decarboxylated by the enzyme.