Primary structure of bovine pituitary secretory protein I (chromogranin A) deduced from the cDNA sequence.
Primary structure of bovine pituitary secretory protein I (chromogranin A) deduced from the cDNA sequence.
复制标题
从 cDNA 序列推导出牛垂体分泌蛋白 I（嗜铬粒蛋白 A）的一级结构。
DOI：
10.1073/pnas.84.14.5043
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发表时间：
1987
影响因子：
11.1
通讯作者：
Levine,MA
中科院分区：
文献类型：
--
作者：
Ahn,TG;Cohn,DV;Gorr,SU;Ornstein,DL;Kashdan,MA;Levine,MA
分泌蛋白I（SP-I），也称为嗜铬粒蛋白A，是一种酸性糖蛋白，其已在所检查的内分泌和神经内分泌来源的每个组织中发现，但从未在外分泌或上皮细胞中发现。它与肽类激素和神经递质的共储存和共分泌表明它具有重要的内分泌功能。我们已经分离的cDNA克隆从牛垂体λ gt 11表达文库使用抗血清甲状旁腺SP-Ⅰ。最大的克隆（SP 4 B）（约等于1.6个核糖核酸酶）与甲状旁腺、垂体和肾上腺髓质RNA中2.1个核糖核酸酶的转录本杂交。SP 4 B溶原菌衍生的细菌裂解物的免疫印迹显示特异性抗体结合SP 4 B/β-半乳糖苷酶融合蛋白（160 kDa），cDNA衍生组分为46 kDa。用SP-I抗血清对细菌裂解物进行放射免疫测定，得到125 I标记的SP-I与SP 4 B裂解物和真实SP-I的平行置换曲线。SP 4 B含有1614个核苷酸的cDNA，其编码449个氨基酸的蛋白质（计算质量，50 kDa）。垂体SP-Ⅰ cDNA和肾上腺髓质SP-Ⅰ cDNA的核苷酸序列几乎相同。基因组DNA分析表明，垂体、肾上腺和甲状旁腺SP-Ⅰ是同一基因的产物。
Secretory protein I (SP-I), also referred to as chromogranin A, is an acidic glycoprotein that has been found in every tissue of endocrine and neuroendocrine origin examined but never in exocrine or epithelial cells. Its co-storage and co-secretion with peptide hormones and neurotransmitters suggest that it has an important endocrine or secretory function. We have isolated cDNA clones from a bovine pituitary lambda gt11 expression library using an antiserum to parathyroid SP-I. The largest clone (SP4B) (approximately equal to 1.6 kilobases) hybridized to a transcript of 2.1 kilobases in RNA from parathyroid, pituitary, and adrenal medulla. Immunoblots of bacterial lysates derived from SP4B lysogens demonstrated specific antibody binding to an SP4B/beta-galactosidase fusion protein (160 kDa) with a cDNA-derived component of 46 kDa. Radioimmunoassay of the bacterial lysates with SP-I antiserum yielded parallel displacement curves of 125I-labeled SP-I by the SP4B lysate and authentic SP-I. SP4B contains a cDNA of 1614 nucleotides that encodes a 449-amino acid protein (calculated mass, 50 kDa). The nucleotide sequences of the pituitary SP-I cDNA and adrenal medullary SP-I cDNAs are nearly identical. Analysis of genomic DNA suggests that pituitary, adrenal, and parathyroid SP-I are products of the same gene.