DISTRIBUTION OF SPARC IN NORMAL AND NEOPLASTIC HUMAN TISSUE
DISTRIBUTION OF SPARC IN NORMAL AND NEOPLASTIC HUMAN TISSUE
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DOI：
10.1177/43.8.7622842
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发表时间：
1995-08-01
影响因子：
3.2
通讯作者：
GOWN, AM
中科院分区：
文献类型：
--
作者：
PORTER, PL;SAGE, EH;GOWN, AM
骨粘连蛋白（分泌蛋白，酸性且富含半胱氨酸）/骨粘连蛋白是一种分泌型糖蛋白，其在小鼠成体和胚胎组织中表现出有限的表达，并且与细胞迁移、基质矿化、类固醇激素产生、细胞周期调节和血管生成相关。我们生产了一种单克隆抗体，单克隆抗体SSP 2，对Ca 2+结合区的ESTA和评估的免疫反应性正常和恶性组织从118人的样本。在正常组织中，我们发现类固醇生成细胞、软骨细胞、胎盘滋养层细胞、血管平滑肌细胞和内皮细胞中存在与SSP 2的有限和中等反应性。在参与组织修复和侵袭性恶性肿瘤（包括胃肠道、乳腺、肺、肾、肾上腺皮质、卵巢和脑的肿瘤）的纤维细胞和内皮细胞中发现了强反应性。我们的结论是，SSP 2是一个有用的试剂，用于检测在人体组织中的病毒。鉴于恶性组织的广泛反应性，我们提出，cytokine表达可能有助于肿瘤进展的某些方面。
SPARC (Secreted Protein, Acidic and Rich in Cysteine)/osteonectin is a secreted glycoprotein that exhibits restricted expression in murine adult and embryonic tissues and is associated with cell migration, matrix mineralization, steroid hormone production, cell cycle regulation, and angiogenesis. We produced a monoclonal antibody, MAb SSP2, against a Ca2+-binding region of SPARC and evaluated the immunoreactivity of normal and malignant tissue from 118 human samples. In normal tissue we found restricted and moderate reactivity with SSP2 in steroidogenic cells, chondrocytes, placental trophoblasts, vascular smooth muscle cells, and endothelial cells. Strong reactivity was found in fibrocytes and endothelial cells involved in tissue repair and in invasive malignant tumors, including those of the gastrointestinal tract, breast, lung, kidney, adrenal cortex, ovary, and brain. We conclude that SSP2 is a useful reagent for detection of SPARC in human tissue. Given the broad reactivity of malignant tissues, we propose that SPARC expression might contribute to some aspects of tumor progression.