Control of OXPHOS efficiency by complex I in brain mitochondria
Control of OXPHOS efficiency by complex I in brain mitochondria
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DOI：
10.1016/j.neurobiolaging.2007.08.002
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发表时间：
2009-04-01
影响因子：
4.2
通讯作者：
Villani, Gaetano
中科院分区：
文献类型：
--
作者：
Cocco, Tiziana;Pacelli, Consiglia;Villani, Gaetano
在目前的工作中，我们分析了大鼠脑，肝和心脏线粒体的氧化磷酸化（OXPHOS）的能量生产的效率（P/O比）。这项研究揭示了组织特异性的P/O比和ATP生产率的平均值的差异。在复合物I（NADH：泛醌氧化还原酶）中观察到P/O比对呼吸速率的显着依赖性，但在复合物II（琥珀酸脱氢酶）呼吸底物中则没有观察到。通过将分析扩展到来自三个独立动物组的脑线粒体，进一步证实了复合物I底物的P/O变化的生理影响，所述三个独立动物组用于研究饮食处理对OXPHOS的年龄相关变化的影响。速率依赖性P/O变异的一般位点特异性表明，解耦，即电子转移和质子泵之间的耦合减少，可能主要是由于线粒体复合物I的滑动。这些研究结果表明，一个额外的机制所发挥的关键作用的节能呼吸复合物I的线粒体OXPHOS的生理和适应性可塑性。(C)2007年爱思唯尔公司All rights reserved.
In the present work we have analysed the efficiency (P/O ratio) of energy production by oxidative phosphorylation (OXPHOS) in rat brain, liver and heart mitochondria. This study has revealed tissue-specific differences in the mean values of P/O ratios and ATP production rates. A marked dependence of the P/O ratio on the respiration rates has been observed with complex I (NADH: ubiquinone oxidoreductase), but not with complex II (succinate dehydrogenase) respiratory substrates. The physiological impact of the P/O variations with complex I substrates has been further confirmed by extending the analysis to brain mitochondria from three independent groups of animals utilized to study the effects of dietary treatments on the age-related changes of OXPHOS. The general site-specificity of the rate-dependent P/O variability indicates that the decoupling, i.e. decreased Coupling between electron transfer and proton pumping, is likely to be mostly due to slip of mitochondrial complex I. These findings suggest an additional mechanism for the pivotal role played by the energy-conserving respiratory complex I in the physiological and adaptive plasticity of mitochondrial OXPHOS. (C) 2007 Elsevier Inc. All rights reserved.