Can Acropora tenuis larvae attract native Symbiodiniaceae cells by green fluorescence at the initial establishment of symbiosis?
Can Acropora tenuis larvae attract native Symbiodiniaceae cells by green fluorescence at the initial establishment of symbiosis?
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DOI：
10.1371/journal.pone.0252514
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Suzuki G
中科院分区：
文献类型：
--
作者：
Yamashita H;Koike K;Shinzato C;Jimbo M;Suzuki G
大多数珊瑚从周围环境中获取共生藻类共生体来启动共生。环境中共生菌科的细胞密度通常较低，可能存在新的珊瑚世代吸引合适的内共生体的机制。珊瑚中合适的共生藻细胞对绿色荧光的趋光性已被认为是这样的机制之一。在本研究中，我们观察了共生科各菌株的趋光作用波长和无共生细角鹿角幼虫在内共生体摄取时的荧光光谱。共生科物种和“天然”内共生体之间的趋光性模式有所不同——常见于自然环境中的鹿角珊瑚幼体；也就是说，小亚德里亚共生藻被蓝光而不是绿光所吸引。另一种天然内共生体，Durusdinium Trenchii，没有表现出对任何波长特异的趋光性。尽管幼虫在蓝紫色激发光下表现出绿色和宽橙色荧光，但最大绿色荧光峰与小亚得里亚海虱的趋光作用光谱并不重合。相反，在幼虫绿色荧光的峰值波长附近，这种天然内共生体表现出轻微的负趋光性，这表明细细蠓幼虫的绿色荧光可能在天然内共生体的初始吸引中不起作用。相反，UV-A 激发下的宽蓝色幼虫荧光覆盖了 S. microadriaticum 的最大趋光作用波长。我们还在不激发可见幼虫荧光的红色 LED 灯下使用天然内共生体和非共生幼虫进行了感染测试。几乎所有幼虫都未能获得 S. microadriaticum 细胞，而 D. Trenchii 细胞即使在红色照明下也能被幼虫获得。因此，可能存在可见荧光以外的吸引机制，至少在 D. Trenchii 的情况下是这样。我们的结果表明，需要进一步的研究和讨论，而不仅仅是绿色荧光，以阐明最初的吸引机制。
Most corals acquire symbiodiniacean symbionts from the surrounding environment to initiate symbiosis. The cell densities of Symbiodiniaceae in the environment are usually low, and mechanisms may exist by which new coral generations attract suitable endosymbionts. Phototaxis of suitable symbiodiniacean cells toward green fluorescence in corals has been proposed as one such mechanism. In the present study, we observed the phototaxis action wavelength of various strains of Symbiodiniaceae and the fluorescence spectra of aposymbiotic Acropora tenuis larvae at the time of endosymbiont uptake. The phototaxis patterns varied among the Symbiodiniaceae species and “native” endosymbionts—commonly found in Acropora juveniles present in natural environments; that is, Symbiodinium microadriaticum was attracted to blue light rather than to green light. Another native endosymbiont, Durusdinium trenchii, showed no phototaxis specific to any wavelength. Although the larvae exhibited green and broad orange fluorescence under blue-violet excitation light, the maximum green fluorescence peak did not coincide with that of the phototaxis action spectrum of S. microadriaticum. Rather, around the peak wavelength of larval green fluorescence, this native endosymbiont showed slightly negative phototaxis, suggesting that the green fluorescence of A. tenuis larvae may not play a role in the initial attraction of native endosymbionts. Conversely, broad blue larval fluorescence under UV-A excitation covered the maximum phototaxis action wavelength of S. microadriaticum. We also conducted infection tests using native endosymbionts and aposymbiotic larvae under red LED light that does not excite visible larval fluorescence. Almost all larvae failed to acquire S. microadriaticum cells, whereas D. trenchii cells were acquired by larvae even under red illumination. Thus, attraction mechanisms other than visible fluorescence might exist, at least in the case of D. trenchii. Our results suggest that further investigation and discussion, not limited to green fluorescence, would be required to elucidate the initial attraction mechanisms.
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影响因子：
3.7
作者：
Alieva NO;Konzen KA;Field SF;Meleshkevitch EA;Hunt ME;Beltran-Ramirez V;Miller DJ;Wiedenmann J;Salih A;Matz MV
通讯作者：
Matz MV
影响因子：
9.2
作者：
LaJeunesse, Todd C.;Parkinson, John Everett;Santos, Scott R.
通讯作者：
Santos, Scott R.
影响因子：
2.4
作者：
Lee, Sung Yeon;Jeong, Hae Jin;Lajeunesse, Todd C.
通讯作者：
Lajeunesse, Todd C.
DOI：
10.1098/rspb.2010.2344
发表时间：
2011-09-07
期刊：
Proceedings. Biological sciences
影响因子：
--
作者：
Kenkel CD;Traylor MR;Wiedenmann J;Salih A;Matz MV
通讯作者：
Matz MV
影响因子：
4.9
作者：
Abrego, David;Van Oppen, Madeleine J. H.;Willis, Bette L.
通讯作者：
Willis, Bette L.