Growth performance, haematological changes, immune response, antioxidant activity and disease resistance in rainbow trout (Oncorhynchus mykiss) fed diet supplemented with ellagic acid.
Growth performance, haematological changes, immune response, antioxidant activity and disease resistance in rainbow trout (Oncorhynchus mykiss) fed diet supplemented with ellagic acid.
复制标题
DOI：
10.1016/j.fsi.2019.10.056
复制
发表时间：
2019-12
影响因子：
4.7
通讯作者：
S. MİŞE YONAR
中科院分区：
文献类型：
--
作者：
S. MİŞE YONAR
研究了不同水平的鞣花酸（EA）对虹鳟（Oncorhynchus mykiss）生长性能、血液学指标、免疫功能、抗鲁氏耶尔森氏菌感染和氧化/抗氧化状态的影响。用对照饲料和三种不同的实验饲料（含有三种不同水平的EA（50，100和200 mg kg-1饲料））喂养鱼8周。在实验结束时，测量生长性能[增重（WG）、特定生长率（SGR）和饲料转化率（FCR）]、血液学值[红细胞（RBC）计数、血红蛋白（Hb）浓度、红细胞压积（Ht）水平和红细胞指数：平均红细胞体积（MCV）、平均红细胞血红蛋白（MCH）和平均红细胞血红蛋白浓度（MCHC）]，免疫应答[白色血细胞（WBC）计数、氧化自由基产生（硝基蓝四唑（NBT）测定）、吞噬活性（PA）和吞噬指数（PI）、总蛋白（TP）和免疫球蛋白M（IgM）水平、血清杀菌活性（BA）、溶菌酶（LYZ）和髓过氧化物酶（MPO）活性]和氧化/抗氧化状态[组织丙二醛（MDA）水平和超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和谷胱甘肽过氧化物酶（GSH-Px）活性]进行分析。此外，鱼被攻击Y。记录14天的Ruckeri和存活率。在与EA喂养组的生长参数，如WG，SGR，和FCR没有显着变化。与对照组相比，电针组的RBC计数、Hb浓度和Ht水平均升高。而各组间MCV、MCH和MCHC值无显著性差异。结果表明，与对照组相比，用EA喂养的组中的所有免疫学参数均增强。用Y. ruckeri揭示了用EA喂养的组中死亡率的降低。与对照组相比，饲粮EA刺激了肝脏、头肾和脾脏中SOD、CAT和GSH-Px活性，但组织中MDA含量呈相反趋势。本研究表明，EA能有效地提高虹鳟鱼的血液学指标、免疫功能、抗氧化能力和抗病能力。
The present study was performed to investigate the effects of various levels of dietary ellagic acid (EA) on growth performance, haematological values, immune response, protection againstYersinia ruckeriinfection, and oxidant/antioxidant status in rainbow trout,Oncorhynchus mykiss. Fish were fed with the control diet and three different experimental diets containing three graded levels of EA (50, 100 and 200 mg kg−1diet) for 8 weeks. At the end of the experiment, the growth performance [weight gain (WG), specific growth rate (SGR) and feed conversion ratio (FCR)], haematological values [the red blood cell (RBC) count, haemoglobin (Hb) concentration, haematocrit (Ht) level and erythrocyte indices: mean corpuscular volume (MCV), mean corpuscular haemoglobin (MCH) and mean corpuscular haemoglobin concentration (MCHC)], immune response [white blood cell (WBC) count, oxidative radical production (nitroblue tetrazolium (NBT) assay), phagocytic activity (PA) and phagocytic index (PI), total protein (TP) and immunoglobulin M (IgM) levels, serum bactericidal activity (BA), lysozyme (LYZ) and myeloperoxidase (MPO) activities] and oxidant/antioxidant status [tissue malondialdehyde (MDA) levels and superoxide dismutase (SOD), catalase (CAT) and glutathione peroxidase (GSH-Px) activities] were analysed. In addition, fish were challenged byY. ruckeriand survival rate was recorded for 14 days. In the groups fed with EA the growth parameters such as WG, SGR, and FCR did not vary significantly. The RBC count, Hb concentration, and Ht level increased in the groups fed with EA when compared with the control group. However, there were no significant differences in the MCV, MCH and MCHC values among the groups. The results demonstrated enhancement in all the immunological parameters in the groups fed with EA compared to the control group. The results obtained from challenge withY. ruckerirevealed reduction in the mortalities in the groups fed with EA. The dietary EA stimulated the SOD, CAT and GSH-Px activities in liver, head kidney and spleen as compared to the control group; however, a reverse trend was observed in the MDA levels of tissues. The present study suggest that EA can effectively enhance the haematological values, immune response, antioxidant capacity, and disease resistance in rainbow trout.